Document Detail


Two tales of antioxidant enzymes on β cells and diabetes.
MedLine Citation:
PMID:  20618069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pancreatic islets contain low activities of catalase, selenium-dependent glutathione peroxidase 1 (GPX1), and Cu,Zn-superoxide dismutase 1 (SOD1). Thus, enhancing expression of these enzymes in islets has been unquestionably favored. However, such an attempt has produced variable metabolic outcomes. While β cell-specific overexpression of Sod1 enhanced mouse resistance to streptozotocin-induced diabetes, the same manipulation of catalase aggravated onset of type 1 diabetes in nonobese diabetic mice. Global overexpression of Gpx1 in mice induced type 2 diabetes-like phenotypes. Although knockouts of Gpx1 and Sod1 each alone or together decreased pancreatic β cell mass and plasma insulin concentrations, these knockouts improved body insulin sensitivity to different extents. Pancreatic duodenal homeobox 1, forkhead box A2, and uncoupling protein 2 are three key regulators of β cell mass, insulin synthesis, and glucose-stimulated insulin secretion. Phenotypes resulted from altering GPX1 and/or SOD1 were partly mediated through these factors, along with protein kinase B and c-jun terminal kinase. A shifted reactive oxygen species inhibition of protein tyrosine phosphatases in insulin signaling might be attributed to altered insulin sensitivity. Overall, metabolic roles of antioxidant enzymes in β cells and diabetes depend on body oxidative status and target functions. Revealing regulatory mechanisms for this type of dual role will help prevent potential pro-diabetic risk of antioxidant over-supplementation to humans.
Authors:
Xin Gen Lei; Marko Z Vatamaniuk
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2010-10-19
Journal Detail:
Title:  Antioxidants & redox signaling     Volume:  14     ISSN:  1557-7716     ISO Abbreviation:  Antioxid. Redox Signal.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-05     Completed Date:  2011-04-14     Revised Date:  2012-02-01    
Medline Journal Info:
Nlm Unique ID:  100888899     Medline TA:  Antioxid Redox Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  489-503     Citation Subset:  IM    
Affiliation:
Department of Animal Science, Cornell University, Ithaca, New York 14853, USA. XL20@cornell.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism*
Catalase / metabolism
Diabetes Mellitus, Type 1 / enzymology*,  physiopathology
Glucose / metabolism
Glutathione Peroxidase / metabolism
Hepatocyte Nuclear Factor 3-beta / metabolism
Homeodomain Proteins / metabolism
Humans
Insulin / metabolism
Insulin-Secreting Cells / enzymology*
Ion Channels / metabolism
Mitochondrial Proteins / metabolism
Oxidative Stress
Reactive Oxygen Species / metabolism
Signal Transduction / physiology
Superoxide Dismutase / metabolism
Trans-Activators / metabolism
Grant Support
ID/Acronym/Agency:
DK53018/DK/NIDDK NIH HHS; R01 DK053018-11/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Homeodomain Proteins; 0/Insulin; 0/Ion Channels; 0/Mitochondrial Proteins; 0/Reactive Oxygen Species; 0/Trans-Activators; 0/mitochondrial uncoupling protein 2; 0/pancreatic and duodenal homeobox 1 protein; 135845-92-0/Hepatocyte Nuclear Factor 3-beta; 50-99-7/Glucose; EC 1.11.1.-/glutathione peroxidase GPX1; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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