Document Detail

Two OPEEs (organic phase enzyme electrodes) used to check the percentage water content in hydrophobic foods and drugs.
MedLine Citation:
PMID:  11763068     Owner:  NLM     Status:  MEDLINE    
The development and optimization of an analytical method using enzymatic biosensors able to operate in organic solvents [organic phase enzyme electrodes (OPEEs)] for the determination of the water content in food fats (butter, margarine) or pharmaceutical or cosmetic ointments is described. The method is based on the increase in enzymatic activity which is related to the increase in the percentage water content in the organic phase into which the biosensor is dipped. The enzymes used to assemble the biosensors were tyrosinase or catalase, the substrates were phenol or p-cresol and tert-butyl hydroperoxide, respectively, and the organic solvents were acetonitrile or dioxane. A gas diffusion amperometric electrode for oxygen measurement was used as electrochemical transducer. The results were compared with those obtained applying the Karl Fischer method to the same food or drug matrices. The correlations among the two methods proved satisfactory, as the difference in the computed values of water content was never higher than 7%. Also, the precision of measurements was acceptable (RSD < 6%) in all the analyses of real matrices.
L Campanella; G De Santis; G Favero; M P Sammartino; M Tomassetti
Related Documents :
21520448 - The effect of postharvest ripening on strawberry bioactive composition and antioxidant ...
7400088 - Acid digestion, hydride evolution atomic absorption spectrophotometric method for deter...
21320368 - A protein-rich beverage consumed as a breakfast meal leads to weaker appetitive and die...
21262268 - Nucleus accumbens dopamine and mu-opioid receptors modulate the reinstatement of food-s...
25422558 - A novel approach for the efficient extraction of silybin from milk thistle fruits.
18963118 - Evaluation of sulphuric acid treatment as the clean-up step for the estimation of bhc a...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Analyst     Volume:  126     ISSN:  0003-2654     ISO Abbreviation:  Analyst     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-12-12     Completed Date:  2002-01-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  1923-8     Citation Subset:  IM    
Department of Chemistry, University of Rome La Sapienza, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biosensing Techniques*
Butter / analysis
Cosmetics / chemistry*
Food Analysis / methods*
Margarine / analysis
Ointments / chemistry
Water / analysis*
Reg. No./Substance:
0/Cosmetics; 0/Ointments; 7732-18-5/Water; 8029-34-3/Butter; 8029-82-1/Margarine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A glucose biosensor based on electrodeposited biocomposites of gold nanoparticles and glucose oxidas...
Next Document:  Effect of interfering substances on current response of recombinant peroxidase and glucose oxidase-r...