Document Detail


Two FAD3 desaturase genes control the level of linolenic acid in flax seed.
MedLine Citation:
PMID:  16113219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Industrial oil flax (Linum usitatissimum) and edible oil or solin flax differ markedly in seed linolenic acid levels. Despite the economic importance of low-linolenic-acid or solin flax, the molecular mechanism underlying this trait has not been established. Two independently inherited genes control the low-linolenic-acid trait in flax. Here, we identified two genes, LuFAD3A and LuFAD3B that encode microsomal desaturases capable of desaturating linoleic acid. The deduced proteins encoded by these genes shared 95.4% identity. In the low-linolenic-acid line solin 593-708, both LuFAD3A and LuFAD3B carry point mutations that produce premature stop codons. Expression of these genes in yeast (Saccharomyces cerevisiae) demonstrated that, while the wild-type proteins were capable of desaturating linoleic acid, the truncated proteins were inactive. Furthermore, the low-linolenic-acid phenotype in flax was complemented by transformation with a wild-type gene. Codominant DNA markers were developed to distinguish between null and wild-type alleles of both genes, and linolenic acid levels cosegregated with genotypes, providing further proof that LuFAD3A and LuFAD3B are the major genes controlling linolenic acid levels in flax. The level of LuFAD3 transcripts in seeds peaked at about 20 d after flowering, and transcripts were not detectable in leaf, root, or stem tissue. A dramatic reduction in transcript levels of both genes occurred in the low-linolenic-acid solin line, which was likely due to nonsense-mediated decay.
Authors:
Patricia Vrinten; Zhiyuan Hu; Mary-Ann Munchinsky; Gordon Rowland; Xiao Qiu
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Publication Detail:
Type:  Journal Article     Date:  2005-08-19
Journal Detail:
Title:  Plant physiology     Volume:  139     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-16     Completed Date:  2006-01-23     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-87     Citation Subset:  IM    
Affiliation:
Bioriginal Food and Science Corporation, Saskatoon, Saskatchewan, Canada.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ116424;  DQ116425
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Flax / enzymology*,  genetics*,  metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genetic Markers
Genotype
Molecular Sequence Data
Phenotype
Plant Proteins / genetics*,  metabolism*
Point Mutation
RNA, Messenger / genetics,  metabolism
Seeds / enzymology,  genetics,  metabolism*
Sequence Homology, Amino Acid
Transcription, Genetic
alpha-Linolenic Acid / metabolism*
Chemical
Reg. No./Substance:
0/Genetic Markers; 0/Plant Proteins; 0/RNA, Messenger; 463-40-1/alpha-Linolenic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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