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Two-Dimensional Differential Gel Electrophoresis of a Cell Line Derived from a Breast Cancer Micrometastasis Revealed a Stem/Progenitor Cell Protein Profile.
MedLine Citation:
PMID:  19215085     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Dissemination of primary cancer cells to distant sites is an early event in breast cancer. These cells can invade the bone marrow, rest there, and many years later disseminated tumor cells (DTC) can grow out to form overt metastases. Epithelium specific cytokeratins are commonly used as marker proteins for sensitive detection of metastatic lesions. However, due to difficulties in the detection of DTC, the question arises if DTC necessarily have the same protein expression profile as advanced tumors. On that account, we analyzed the previously uncharacterized breast cancer DTC cell line BC-M1 by 2-D DIGE. Special protein concentration and purification protocols for 2-DE were developed which resulted in high recovery rates and increased display of alkaline proteins. A broad range reference map of metastasis relevant proteins was compiled including the cytokeratins 5, 7, 8, 17, 18, and 19 and several classes of cytoskeleton proteins involved in metastasis like ezrin, gelsolin, vinculin, or vimentin. BC-M1 shows the rare and highly metastatic vimentin/cytokeratin 5 positive and cytokeratin 8/18 negative breast cancer phenotype and expresses Her-2, which is also found in stem cells/progenitor cells of primary tumors. Supported by the detection of several other epithelium-derived proteins, the example BC-M1 indicates that the protein expression profile of DTC might be reminiscent of the expression profile of the early tumor, which differs from the advanced tumor. Hence, DTC from breast cancer patients' bone marrow expressed cytokeratin 5, which further supports our hypothesis.
Authors:
Kai Bartkowiak; Marek Wieczorek; Friedrich Buck; Sönke Harder; Jennifer Moldenhauer; Katharina E Effenberger; Klaus Pantel; Jasna Peter-Katalinic; Burkhard H Brandt
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2009-2-12
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-2-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute for Tumor Biology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany, Institute of Clinical Chemistry, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany, Department of Plant Biochemistry and Biotechnology, Westphalian Wilhelm's-University Munster, Hindenburgplatz 55, 48143 Munster, Germany, and Institute of Medical Physics and Biophysics, Westphalian Wilhelm's-University Munster, Robert-Koch Str. 31, D-48149 Munster, Germany.
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