Document Detail


Two 2[5H]-furanones as possible signaling molecules in Lactobacillus helveticus.
MedLine Citation:
PMID:  16957229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two 2[5H]-furanones, in association with medium-chain fatty acids, were released in whey by Lactobacillus helveticus exposed to oxidative and heat stresses. This species plays an important role in cheese technology, particularly for Swiss-type cheeses and Grana cheese. Moreover, it significantly contributes to cheese ripening by means of an early autolysis and the release of enzymes during processing. Experimental evidence of the involvement of the two 2[5H]-furanones, detected by a gas chromatography-mass spectrometry/solid-phase microextraction technique, in the autolysis phenomenon has been obtained. Zymograms performed by using renaturing sodium dodecyl sulfate-polyacrylamide gels were used to detect the bioactivity of the supernatants containing the two furanones on fresh cells of the same strain. In addition to bands corresponding to known autolysins, new autolysins were detected concomitant with the exposure of Lactobacillus helveticus to the supernatants, which can be regarded as conditioned media (CM), and to a commercial furanone, 5-ethyl-3-hydroxy-4-methyl-2[5H]-furanone (HEMFi), having spectral data similar to those of the newly described 2[5H]-furanones. Morphological changes were observed when fresh cells were exposed to CM containing the two 2[5H]-furanones and HEMFi. The two furanones produced by Lactobacillus helveticus, which met a number of criteria to be included in cell-cell signaling molecules, have a presumptive molecular mass lower than those of already known 3[2H]-furanones having an autolytic activity and being produced by gram-negative bacteria. Moreover, they present a different chemical structure with respect to the furanones already identified as products of Lactococcus lactis subsp. cremoris or to those identified in some cheeses with Lactobacillus helveticus as a starter culture.
Authors:
Maurice Ndagijimana; Melania Vallicelli; P Sandro Cocconcelli; Fabrizio Cappa; Francesca Patrignani; Rosalba Lanciotti; M Elisabetta Guerzoni
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-07     Completed Date:  2006-11-08     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6053-61     Citation Subset:  IM    
Affiliation:
Dipartimento Scienze degli Alimenti, Alma Mater Studiorum, University of Bologna, Via Fanin 46, 40127 Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Cheese / microbiology
Culture Media, Conditioned
Food Microbiology
Furans / chemistry,  metabolism*,  pharmacology
Gas Chromatography-Mass Spectrometry
Hydrogen Peroxide / pharmacology
Lactobacillus helveticus / drug effects,  metabolism*,  ultrastructure
Microscopy, Electron, Scanning
Molecular Structure
N-Acetylmuramoyl-L-alanine Amidase / metabolism
Signal Transduction
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Furans; 7722-84-1/Hydrogen Peroxide; EC 3.5.1.28/N-Acetylmuramoyl-L-alanine Amidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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