Document Detail

Twenty-eight-day bed rest with hypercortisolemia induces peripheral insulin resistance and increases intramuscular triglycerides.
MedLine Citation:
PMID:  19919871     Owner:  NLM     Status:  MEDLINE    
Spaceflight represents a unique physiologic challenge to humans, altering hormonal profiles and tissue insulin sensitivity. Among these hormonal alterations, hypercortisolemia and insulin insensitivity are thought to negatively affect muscle mass and function with spaceflight. As insulin sensitivity influences the accumulation of muscle triglycerides, we examined this relationship during hypercortisolemia and inactivity. Six young healthy volunteers were confined to bed rest for 28 days. To mimic the stress response observed during spaceflight, hypercortisolemia (20-24 mg/dL) was induced and maintained by oral ingestion of hydrocortisone. On days 1 and 28 of bed rest, insulin sensitivity across the leg was assessed with a local (femoral arterial insulin infusion) 2-stage hyperinsulinemic-euglycemic clamp (stage 1, 35 microU/min per milliliter of leg; stage 2, 70 microU/min per milliliter of leg). Intramuscular lipid was measured with magnetic resonance spectroscopy. After bed rest, there was a decrease in insulin sensitivity, as assessed by glucose uptake during hyperinsulinemia (from 9.1 +/- 1.3 [mean +/- SEM] to 5.2 +/- 0.7 mg/kg of leg per minute [P = .015]). Intramuscular triglyceride increased from 0.077 +/- 0.011 to 0.136 +/- 0.018 (signal area of fat/signal area of standard, P = .009). Intramuscular lipid content correlated with the glucose uptake at day 28 (R = -0.85, P = .035). These data demonstrate that muscular inactivity and hypercortisolemia are associated with an increase in intramuscular triglyceride and skeletal muscle insulin resistance in previously healthy subjects.
Melanie G Cree; Douglas Paddon-Jones; Bradley R Newcomer; Ola Ronsen; Asle Aarsland; Robert R Wolfe; Arny Ferrando
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-11-17
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  59     ISSN:  1532-8600     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-05-03     Revised Date:  2014-07-17    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  United States    
Other Details:
Languages:  eng     Pagination:  703-10     Citation Subset:  IM    
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MeSH Terms
Bed Rest / adverse effects*
Blood Glucose / analysis,  metabolism
Body Weight / physiology
Cushing Syndrome / blood,  metabolism*
Fatty Acids, Nonesterified / blood
Glucose Clamp Technique
Glycerol / blood
Insulin / blood,  metabolism
Insulin Resistance / physiology*
Linear Models
Muscle, Skeletal / metabolism*
Triglycerides / metabolism*
Grant Support
5 R01 GM 57295/GM/NIGMS NIH HHS; M01 RR 00073/RR/NCRR NIH HHS; P30 AG024832/AG/NIA NIH HHS; R01 GM057295-07/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Blood Glucose; 0/Fatty Acids, Nonesterified; 0/Insulin; 0/Triglycerides; PDC6A3C0OX/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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