| Twelve-week RBC storage. | |
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MedLine Citation:
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PMID: 12823745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Better storage can improve RBC availability and safety. Optimizing RBC ATP production and minimizing hemolysis has allowed progressively longer storage. STUDY DESIGN AND METHODS: In the first study, 24 units of packed CPD RBCs were pooled in groups of four, realiquoted, and added to 300 mL of one of four variants of experimental additive solution 76 (EAS-76) containing 45, 40, 35, or 30 mEq per L NaCl. Units were sampled weekly for 12 weeks for morphologic and biochemical measures. In the second study, 10 volunteers donated 2 units of RBCs for a crossover comparison of Tc/Cr 24-hour in vivo recovery of 6-week storage in AS-1 versus 12-week storage in EAS-76 variant 6 (EAS-76v6) having 30 mEq per L NaCl. RESULTS: RBCs stored in the lower salt variants of EAS-76 had higher concentrations of RBC ATP with less hemolysis and microvesiculation. RBC 2,3 DPG was preserved for two weeks. RBCs stored for 12 weeks in EAS-76v6 exhibited 78 +/- 4 percent 24-hour in vivo recovery. CONCLUSIONS: It is possible to store RBCs for 12 weeks with acceptable recovery and 0.6 percent hemolysis and with normal 2,3 DPG concentrations for 2 weeks. |
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Authors:
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John R Hess; Heather R Hill; Cindy K Oliver; Lloyd E Lippert; Neeta Rugg; Amy D Joines; Jennifer F Gormas; P Gayle Pratt; Edward B Silverstein; Tibor J Greenwalt |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Transfusion Volume: 43 ISSN: 0041-1132 ISO Abbreviation: Transfusion Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-06-25 Completed Date: 2003-07-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 867-72 Citation Subset: IM |
Affiliation:
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Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC, USA. jhess@umm.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,3-Diphosphoglycerate
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blood Adenosine Triphosphate / blood Blood Donors Blood Preservation* / methods Erythrocyte Transfusion Erythrocytes* Female Hemolysis Humans Male Sodium Chloride / analysis Solutions Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Solutions; 138-81-8/2,3-Diphosphoglycerate; 56-65-5/Adenosine Triphosphate; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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