Document Detail

Twelve-week RBC storage.
MedLine Citation:
PMID:  12823745     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Better storage can improve RBC availability and safety. Optimizing RBC ATP production and minimizing hemolysis has allowed progressively longer storage. STUDY DESIGN AND METHODS: In the first study, 24 units of packed CPD RBCs were pooled in groups of four, realiquoted, and added to 300 mL of one of four variants of experimental additive solution 76 (EAS-76) containing 45, 40, 35, or 30 mEq per L NaCl. Units were sampled weekly for 12 weeks for morphologic and biochemical measures. In the second study, 10 volunteers donated 2 units of RBCs for a crossover comparison of Tc/Cr 24-hour in vivo recovery of 6-week storage in AS-1 versus 12-week storage in EAS-76 variant 6 (EAS-76v6) having 30 mEq per L NaCl. RESULTS: RBCs stored in the lower salt variants of EAS-76 had higher concentrations of RBC ATP with less hemolysis and microvesiculation. RBC 2,3 DPG was preserved for two weeks. RBCs stored for 12 weeks in EAS-76v6 exhibited 78 +/- 4 percent 24-hour in vivo recovery. CONCLUSIONS: It is possible to store RBCs for 12 weeks with acceptable recovery and 0.6 percent hemolysis and with normal 2,3 DPG concentrations for 2 weeks.
John R Hess; Heather R Hill; Cindy K Oliver; Lloyd E Lippert; Neeta Rugg; Amy D Joines; Jennifer F Gormas; P Gayle Pratt; Edward B Silverstein; Tibor J Greenwalt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Transfusion     Volume:  43     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-25     Completed Date:  2003-07-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  867-72     Citation Subset:  IM    
Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC, USA.
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MeSH Terms
2,3-Diphosphoglycerate / blood
Adenosine Triphosphate / blood
Blood Donors
Blood Preservation* / methods
Erythrocyte Transfusion
Sodium Chloride / analysis
Time Factors
Reg. No./Substance:
0/Solutions; 138-81-8/2,3-Diphosphoglycerate; 56-65-5/Adenosine Triphosphate; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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