| Turnover and spreading of old wall during surface growth of Bacillus subtilis. | |
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MedLine Citation:
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PMID: 815238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The steady-state concentration of cell wall turnover products in the medium of Bacillus subtilis 168 growing exponentially on a casein hydrolysate-supplemented medium is equivalent to an overall rate of turnover of less than 10% per generation. After transfer of a steady-labeled culture to nonradioactive medium, the rate of release of labeled turnover products increased exponentially for up to two generations. The rate of turnover finally attained by this culture reached an apparently first-order rate of about 50% per generation. The addition of soluble autolytic activity to growing cultures of a mutant possessing a reduced rate of wall turnover resulted in a marked stimulation in the rate of solubilization of the cell wall fraction. The increased rate of solubilization produced was proportional to the concentration of added enzyme and remained constant until less than 20% of the wall originally present was left. Autolytic activity added under these conditions was bound entirely to wall at least one generation old. The results are interpreted in terms of a model for cell wall growth in which wall two or more generations old covers a total surface area at least four times larger than that occupied at the time of synthesis, forming a shallow outer layer (overlying newer wall) from which all turnover takes place. The model is discussed in relation to previous attempts to determine the pattern of surface expansion in bacilli. |
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Authors:
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H M Pooley |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 125 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1976 Mar |
Date Detail:
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Created Date: 1976-06-02 Completed Date: 1976-06-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1127-38 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylglucosamine
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metabolism Amidohydrolases / metabolism Bacillus subtilis / enzymology, growth & development, metabolism* Bacterial Proteins / biosynthesis Hydrolysis Models, Biological Mucoproteins / biosynthesis Muramidase / metabolism Mutation Peptidoglycan / biosynthesis Subcellular Fractions / metabolism Trypsin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Mucoproteins; 0/Peptidoglycan; 7512-17-6/Acetylglucosamine; EC 3.2.1.17/Muramidase; EC 3.4.21.4/Trypsin; EC 3.5.-/Amidohydrolases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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