Document Detail

Turnover of primary transcripts is a major step in the regulation of mouse H19 gene expression.
MedLine Citation:
PMID:  12151337     Owner:  NLM     Status:  MEDLINE    
In the gene expression pathway, RNA biogenesis is a central multi-step process where both message fidelity and steady-state levels of the mature RNA have to be ascertained. An emerging question is whether RNA levels could be regulated at the precursor stage. Until recently, because it was technically very difficult to determine the level of a pre-mRNA, discrimination between changes in transcriptional activity and in pre-mRNA metabolism was extremely difficult. H19 RNA, the untranslated product of an imprinted gene, undergoes post-transcriptional regulation. Here, using a quantitative real-time RT-PCR approach, we accurately quantify its precursor RNA levels and compare these with the transcriptional activity of the gene, assessed by run-on assays. We find that the levels of H19 precursor RNA are regulated during physiological processes and this regulation appears to be related to RNA polymerase II transcription termination. Our results provide direct evidence that turnover of polymerase II primary transcripts can regulate gene expression in mammals.
Laura Milligan; Thierry Forné; Etienne Antoine; Michaël Weber; Bénédicte Hémonnot; Luisa Dandolo; Claude Brunel; Guy Cathala
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-07-15
Journal Detail:
Title:  EMBO reports     Volume:  3     ISSN:  1469-221X     ISO Abbreviation:  EMBO Rep.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-01     Completed Date:  2003-02-24     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  774-9     Citation Subset:  IM    
Institut de Génétique Moléculaire, UMR 5535 CNRS-Université Montpellier II, France.
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MeSH Terms
Blotting, Northern
Cell Differentiation
Cell Nucleus / metabolism
Cycloheximide / pharmacology
Gene Expression Regulation, Developmental
Genomic Imprinting
Heart / embryology
Protein Synthesis Inhibitors / pharmacology
RNA Processing, Post-Transcriptional
RNA, Long Untranslated
RNA, Messenger / metabolism
RNA, Untranslated / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Reg. No./Substance:
0/H19 long non-coding RNA; 0/Protein Synthesis Inhibitors; 0/RNA, Long Untranslated; 0/RNA, Messenger; 0/RNA, Untranslated; 66-81-9/Cycloheximide

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