Document Detail


Turning a spermatogenic wave into a tsunami: synchronizing murine spermatogenesis using WIN 18,446.
MedLine Citation:
PMID:  23284139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The BDADs (bis-[dichloroacetyl]-diamines) are compounds that can inhibit spermatogenesis via blocking the metabolism of vitamin A. We utilized one specific BDAD, WIN 18,446, to manipulate the endogenous production of retinoic acid (RA) in the testis to further investigate the action of this compound on mammalian sperm production. Transient treatment of adult male mice with WIN 18,446 blocked spermatogonial differentiation and induced significant changes in the cycle of the seminiferous epithelium. WIN 18,446 treatment of neonatal mice also blocked spermatogonial differentiation and, followed by injection of RA, induced synchronous spermatogenesis in adulthood. The net result was pulsatile, rather than normal continuous, release of sperm from the seminiferous epithelium. This study describes a novel technique that can enrich for specific germ cell populations within the testis, representing a valuable new tool for studying spermatogenesis.
Authors:
Cathryn A Hogarth; Ryan Evanoff; Debra Mitchell; Travis Kent; Christopher Small; John K Amory; Michael D Griswold
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-02-14
Journal Detail:
Title:  Biology of reproduction     Volume:  88     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-15     Completed Date:  2013-08-12     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / drug effects,  physiology
Diamines / pharmacology*
Male
Mice
Models, Animal
Spermatogenesis / drug effects*,  physiology*
Testis / cytology,  embryology,  metabolism
Tretinoin / metabolism
Grant Support
ID/Acronym/Agency:
HD 10808/HD/NICHD NIH HHS; R01 HD010808/HD/NICHD NIH HHS; U01 HD060408/HD/NICHD NIH HHS; U54 42454//PHS HHS
Chemical
Reg. No./Substance:
0/Diamines; 1477-57-2/N,N'-bis(dichloroacetyl)-1,8-octamethylenediamine; 5688UTC01R/Tretinoin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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