| Turnover of nonessential fatty acids in cardiolipin from the rat heart. | |
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MedLine Citation:
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PMID: 21957203 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cardiolipin (CL) is a unique phospholipid (PL) found in the mitochondria of mammalian cells. CL remodeling is accompanied by turnover of its fatty acid acyl groups. Abnormalities in CL remodeling have been found in Barth's syndrome, diabetes, and obesity. The objective of this study was to determine nonessential fatty acid turnover in CL and phosphatidylethanolamine (PE) in the rat heart in vivo. Sprague-Dawley rats were fed either a regular chow or a high-fat diet for 15 weeks, and consumed 6% deuterium-enriched drinking water as a tracer for 14 days. CL and PE were extracted from cardiac tissue and isolated by TLC. Fatty acids from CL, PE, and plasma were analyzed by GC/MS for deuterium incorporation. Results showed oleate and vaccenate turnover were the highest in CL whereas palmitate and stearate turnover were low. Among the nonessential fatty acids in PE, turnover of stearate and vaccenate were the highest. The high turnover rate in vaccenate was unexpected, because vaccenate previously had no known metabolic or physiologic function. In conclusion, the similarly high turnover rates of both oleate and vaccenate readily suggest that remodeling is an important functional aspect of PL metabolism in CL. |
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Authors:
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Paulin N Wahjudi; Jennifer K Yee; Steven R Martinez; Jin Zhang; Michael Teitell; Liana Nikolaenko; Ronald Swerdloff; Christina Wang; W N Paul Lee |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-09-27 |
Journal Detail:
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Title: Journal of lipid research Volume: 52 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-14 Completed Date: 2012-03-19 Revised Date: 2012-03-19 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 2226-33 Citation Subset: IM |
Affiliation:
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LA Biomedical Research Institute, Torrance, CA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Animals Cardiolipins / chemistry*, metabolism* Deuterium / chemistry Diet, High-Fat Fatty Acids, Nonesterified / metabolism* Gene Expression Regulation, Enzymologic Kinetics Male Myocardium / metabolism* Phosphatidylethanolamines / metabolism Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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1UL1-RR033176/RR/NCRR NIH HHS; K23 DK083241/DK/NIDDK NIH HHS; M01-RR00425/RR/NCRR NIH HHS; P01 AT003960/AT/NCCAM NIH HHS; R01 CA090571-11/CA/NCI NIH HHS; R01 CA156674-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Cardiolipins; 0/Fatty Acids, Nonesterified; 0/Phosphatidylethanolamines; 39382-08-6/phosphatidylethanolamine; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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