| Turn-on Aptameric System for Simple and Selective Detection of Protein via Base Stacking-dependent DNA Hybridization Event. | |
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MedLine Citation:
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PMID: 21707048 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Base stacking is employed in an entirely new type of sensing platform for the simple and robust detection of protein. Only in the presence of protein, the aptamer DNA can hybridize stably with the capture DNA to form a stem-loop structure due to the enhancement of base stacking. This leads to a strong chemiluminescence emission for simple protein detection. Using platelet-derived growth factor as a model, a fM detection limit was obtained with a dynamic range that spanned four orders of magnitude. Upon modification, the approach presented herein was also sucessfully extended to detect other types of targets including Hg<sup>2+</sup> ion and adenosine and also other types of labels such as fluorescence nanogold. We believe such advancements will represent a significant step toward improved diagnostics and more personalized medical treatment and environmental monitoring. |
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Authors:
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Sheng Cai; Choiwan Lau; Jianzhong Lu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-27 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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