Document Detail

Turku sugar studies. VIII. Principal microbiological findings.
MedLine Citation:
PMID:  1070216     Owner:  NLM     Status:  MEDLINE    
The possible qualitative and/or quantitative alterations in the proportions of cultivable groups of oral microorganisms were analysed during a clinical trial involving the consumption of fructose (F) or xylitol (X) in comparison to sucrose (S). Supragingival plaque samples and paraffin-stimulated saliva were collected from 115 subjects. The samples were dispersed by sonication, diluted stepwise, plated on blood sugar, Mac Leod agar, Mac Concey agar, Rogosa S.L. agar, and Sabouraud agar plates and incubated anerobically and/or aerobically. The number of the total colony forming units (CFU) on blood agar plates in anaerobic incubation was about 1-3 X 10(9)/ml saliva and 1-4 X 10(8)/mg plaque and in aerobic respectively 5-18 X 10(8)/ml saliva and 10(8)/mg plaque. The total CFU on Mac Leod agar was of a similar order of magnitude. The variation between subjects and consecutive determinations was of a similar order of magnitude. The variation between subjects and consecutive determinations was relatively large. The arithmetic mean of the total CFU on Mac Concey agar was about 1-5 X 10(5)/ml saliva, on Rogosa S.L. agar 6-130 X 10(3)/ml saliva and on Sabouraud about 1-2 X 10(3)ml saliva, all in aerobic incubations. Replacement of dietary sucrose with xylitol did not affect the proportion of major microbial categories in saliva or dental plaque. The percentage of typical streptococcal colonies on blood agar was of a similar order of magnitude (about 60-70%) during the diets. The arithmetic and geometric means of the total CFU values on Rogosa and Sabouraud agar plates were significantly lower in the X-group than in the S- or F-groups after a diet period of some months. It was thought that the reason for the reduction of acidogenic and aciduric oral flora in the X-group was partly due to the fact that xylitol is generally not metabolized by these microorganisms.
M Larmas; K K Mäkinen; A Scheinin
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta odontologica Scandinavica     Volume:  34     ISSN:  0001-6357     ISO Abbreviation:  Acta Odontol. Scand.     Publication Date:  1976  
Date Detail:
Created Date:  1977-02-24     Completed Date:  1977-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370344     Medline TA:  Acta Odontol Scand     Country:  FINLAND    
Other Details:
Languages:  eng     Pagination:  285-328     Citation Subset:  D; IM    
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MeSH Terms
Cell Division
Culture Media
Dental Plaque / immunology,  microbiology*
Diet, Cariogenic
Saliva / immunology,  microbiology*
Specimen Handling
Reg. No./Substance:
0/Culture Media; 30237-26-4/Fructose; 57-50-1/Sucrose; 87-99-0/Xylitol; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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