Document Detail


Tuning physical properties of nanocomplexes through microfluidics-assisted confinement.
MedLine Citation:
PMID:  21506589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The future of genetic medicine hinges on successful intracellular delivery of nucleic acid-based therapeutics. While significant effort has concentrated on developing nanocarriers to improve the delivery aspects, scant attention has been paid to the synthetic process of poorly controlled nanocomplex formation. Proposed here is a reliable system to better control the complexation process, and thus the physical properties of the nanocomplexes, through microfluidics-assisted confinement (MAC) in picoliter droplets. We show that these homogeneous MAC-synthesized nanocomplexes exhibit narrower size distribution, lower cytotoxicity, and higher transfection efficiency compared to their bulk-synthesized counterparts. MAC represents a physical approach to control the energetic self-assembly of polyelectrolytes, thereby complementing the chemical innovations in nanocarrier design to optimize nucleic acid and peptide delivery.
Authors:
Yi-Ping Ho; Christopher L Grigsby; Feng Zhao; Kam W Leong
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-20
Journal Detail:
Title:  Nano letters     Volume:  11     ISSN:  1530-6992     ISO Abbreviation:  Nano Lett.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-11     Completed Date:  2011-10-14     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101088070     Medline TA:  Nano Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2178-82     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, United States.
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MeSH Terms
Descriptor/Qualifier:
Colloids / chemistry
DNA / chemistry
Electrolytes
Gene Transfer Techniques
Kinetics
Materials Testing
Microfluidics*
Nanostructures / chemistry*
Nanotechnology / methods*
Nucleic Acids / chemistry
Physics / methods
RNA / chemistry
Grant Support
ID/Acronym/Agency:
HL89764/HL/NHLBI NIH HHS; R01 HL089764-03/HL/NHLBI NIH HHS; R01 HL089764-04/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Colloids; 0/Electrolytes; 0/Nucleic Acids; 63231-63-0/RNA; 9007-49-2/DNA
Comments/Corrections

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