Document Detail

Tunicamycin and Brefeldin A induce in plant cells a programmed cell death showing apoptotic features.
MedLine Citation:
PMID:  11732194     Owner:  NLM     Status:  MEDLINE    
The recent identification of DAD (defender against apoptotic death) gene in plants suggests that the N-linked glycosylation of proteins could be an important control point of plant programmed cell death. In this paper we describe the effects of Tunicamycin, an inhibitor of N-linked protein glycosylation, and Brefeldin A, an inhibitor of protein trafficking from the Golgi apparatus, on sycamore (Acer pseudoplatanus L.) cell cultures. These two chemicals proved able to induce a strong acceleration of the cell death; changes in cell and nucleus morphology; an increase in DNA fragmentation, detectable by a specific immunological reaction; and the presence of oligonucleosomal-size fragments (laddering) in DNA gel electrophoresis. Moreover, Brefeldin A, but not Tunicamycin, strongly stimulated the production of hydrogen peroxide. These results indicate that also in plants chemicals interfering with the activities of endoplasmic reticulum and of Golgi apparatus strongly induce a form of programmed cell death showing apoptotic features.
P Crosti; M Malerba; R Bianchetti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Protoplasma     Volume:  216     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-04     Completed Date:  2002-02-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  31-8     Citation Subset:  IM    
Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, via Celoria, 26, I-20133 Milano, Italy.
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MeSH Terms
Anti-Bacterial Agents / pharmacology
Brefeldin A / pharmacology*
Cell Survival / drug effects
Cells, Cultured
DNA Fragmentation
Hydrogen Peroxide / metabolism
In Situ Nick-End Labeling
Oxidants / metabolism
Protein Synthesis Inhibitors / pharmacology
Sapindaceae / cytology*,  drug effects,  growth & development,  physiology
Tunicamycin / pharmacology*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Oxidants; 0/Protein Synthesis Inhibitors; 11089-65-9/Tunicamycin; 20350-15-6/Brefeldin A; 7722-84-1/Hydrogen Peroxide

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