| Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. | |
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MedLine Citation:
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PMID: 16920778 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Seeds of the tung tree (Vernicia fordii) produce large quantities of triacylglycerols (TAGs) containing approximately 80% eleostearic acid, an unusual conjugated fatty acid. We present a comparative analysis of the genetic, functional, and cellular properties of tung type 1 and type 2 diacylglycerol acyltransferases (DGAT1 and DGAT2), two unrelated enzymes that catalyze the committed step in TAG biosynthesis. We show that both enzymes are encoded by single genes and that DGAT1 is expressed at similar levels in various organs, whereas DGAT2 is strongly induced in developing seeds at the onset of oil biosynthesis. Expression of DGAT1 and DGAT2 in yeast produced different types and proportions of TAGs containing eleostearic acid, with DGAT2 possessing an enhanced propensity for the synthesis of trieleostearin, the main component of tung oil. Both DGAT1 and DGAT2 are located in distinct, dynamic regions of the endoplasmic reticulum (ER), and surprisingly, these regions do not overlap. Furthermore, although both DGAT1 and DGAT2 contain a similar C-terminal pentapeptide ER retrieval motif, this motif alone is not sufficient for their localization to specific regions of the ER. These data suggest that DGAT1 and DGAT2 have nonredundant functions in plants and that the production of storage oils, including those containing unusual fatty acids, occurs in distinct ER subdomains. |
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Authors:
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Jay M Shockey; Satinder K Gidda; Dorselyn C Chapital; Jui-Chang Kuan; Preetinder K Dhanoa; John M Bland; Steven J Rothstein; Robert T Mullen; John M Dyer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-08-18 |
Journal Detail:
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Title: The Plant cell Volume: 18 ISSN: 1040-4651 ISO Abbreviation: Plant Cell Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-13 Completed Date: 2006-11-14 Revised Date: 2010-09-15 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2294-313 Citation Subset: IM |
Affiliation:
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U.S. Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, Louisiana 70124, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF031427; AF129003; AF155224; AF164434; AF384163; AJ131831; AY578727; CAA05274; DQ356679; DQ356680; DQ356681; DQ356682; T45783; TC208469; TC231740; TC251130; TC252055; RefSeq/NM_012079 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Amino Acid Sequence Diacylglycerol O-Acyltransferase / analysis, chemistry, physiology* Endoplasmic Reticulum / enzymology* Euphorbiaceae / enzymology*, genetics Flowers / enzymology, genetics Linolenic Acids / metabolism Molecular Sequence Data Multigene Family Phylogeny Plant Leaves / enzymology, genetics Plant Oils / chemistry Plants, Genetically Modified / cytology, metabolism, ultrastructure Protein Transport / genetics Seeds / enzymology, genetics Sequence Alignment Substrate Specificity Tobacco / cytology, genetics Triglycerides / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Linolenic Acids; 0/Plant Oils; 0/Triglycerides; 13296-76-9/eleostearic acid; EC 2.3.1.20/Diacylglycerol O-Acyltransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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