Document Detail

Tumour necrosis factor-alpha and nitric oxide, determined as nitrite, in malignant pleural effusion.
MedLine Citation:
PMID:  7846335     Owner:  NLM     Status:  MEDLINE    
Pleurodesis treatment is often used to stop chronic pleural effusion in malignant pleurisy. During the treatment a strong intrapleural inflammation is induced leading to the cessation of exudation. In this study the concentrations of tumour necrosis factor (TNF-alpha) and nitric oxide (NO), determined as its immediate metabolite nitrite, were measured in pleural fluid, in order to investigate if they were involved in pleural inflammation and pleural fluid exudation. Determinations were made in pleural fluid from patients with malignant pleural effusion, before and during therapeutic quinacrine-induced pleural inflammation. TNF-alpha concentrations were measured by ELISA-technique and NO/nitrite by chemiluminescence. TNF-alpha increased significantly after quinacrine instillation. NO/nitrite was initially present at the same concentrations as found in normal serum, and increased significantly during treatment. Concentrations of TNF-alpha correlate with the amount of pleural fluid production after quinacrine instillation, in that a higher level of TNF-alpha is associated with more pleural fluid production. No correlation was found between TNF-alpha and NO/nitrite, whereas a weak but significant correlation was found between NO/nitrite and concentrations of another cytokine, interleukin-1 beta (IL-1 beta). The results suggest that TNF-alpha might contribute to pleural fluid exudation and that TNF-alpha and NO are of importance in induced pleural inflammation. IL-1 beta might be one of several stimuli for NO formation in this setting.
V Agrenius; L E Gustafsson; O Widström
Related Documents :
9703945 - Tumor necrosis factor-alpha and -beta upregulate the levels of osteoprotegerin mrna in ...
10432295 - S-adenosylmethionine attenuates the lipopolysaccharide-induced expression of the gene f...
17578885 - Macrophage tnf-alpha contributes to insulin resistance and hepatic steatosis in diet-in...
16272345 - The mechanism of superantigen-mediated toxic shock: not a simple th1 cytokine storm.
2466775 - Alpha-fetoprotein and phagocytosis in athymic nude mice.
3402335 - Aminotransferase activity in the liver of diabetic mice.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Respiratory medicine     Volume:  88     ISSN:  0954-6111     ISO Abbreviation:  Respir Med     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-03-08     Completed Date:  1995-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908438     Medline TA:  Respir Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  743-8     Citation Subset:  IM    
Department of Thoracic Medicine, Karolinska Hospital, Stockholm, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chemiluminescent Measurements
Enzyme-Linked Immunosorbent Assay
Middle Aged
Nitric Oxide / analysis*
Pleural Effusion, Malignant / chemistry*,  therapy
Quinacrine / administration & dosage
Tumor Necrosis Factor-alpha / analysis*
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 83-89-6/Quinacrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Breathlessness in microvascular angina.
Next Document:  Transbronchial lung biopsy through the fibre optic bronchoscope. Results and complications in 452 ex...