Document Detail


Tumorigenic conversion of xeroderma pigmentosum lymphoblastoid cells without karyotypic alteration.
MedLine Citation:
PMID:  2793236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to examine the process of malignant transformation of human somatic cells, we studied the tumorigenic conversion of an Epstein-Barr-virus-immortalized lymphoblastoid cell line (LCL) derived from a patient with xeroderma pigmentosum (XP) complementation group A. Repeated irradiation of the XP cells, XP7NI, with UV-light and subsequent treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in the acquisition of tumorigenicity in athymic nude mice. The tumorigenicity of XP7NI cells was also induced by TPA treatment alone. The tumors formed in athymic mice were of B-cell lymphoma with characteristic histology, cell surface immunoglobulins and an antigen as detected by a B-cell-specific monoclonal antibody (MAb), CD20. The surface immunoglobulins and the HLA type of these tumor cells were identical with those of the parental cells. These malignantly transformed cells retained the same UV sensitivity, serum requirement, colony-forming ability in soft agar, and normal human karyotype as the parental cells. Unlike other tumorigenic lymphoblastoid cell lines, this XP lymphoblastoid cell line provides a unique case in that process(es) leading to tumorigenicity may be induced by UV and TPA without apparent karyotypic changes.
Authors:
A Tachibana; M Toyoda; H Mitani; M S Sasaki; I Arita; H Takebe; K Tatsumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  44     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-11-21     Completed Date:  1989-11-21     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  629-33     Citation Subset:  IM    
Affiliation:
Department of Molecular Oncology, Faculty of Medicine, Kyoto University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Transformation, Neoplastic / drug effects,  pathology*,  radiation effects
Cells, Cultured
Humans
Karyotyping
Lymphocyte Transfusion
Lymphocytes / drug effects,  pathology*,  radiation effects
Mice
Mice, Inbred BALB C
Mice, Nude
Skin Neoplasms / etiology,  pathology*
Tetradecanoylphorbol Acetate / pharmacology
Time Factors
Tumor Stem Cell Assay
Ultraviolet Rays
Xeroderma Pigmentosum / complications,  pathology*
Chemical
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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