Document Detail

Tumor-specific colonization, tissue distribution, and gene induction by probiotic Escherichia coli Nissle 1917 in live mice.
MedLine Citation:
PMID:  17448724     Owner:  NLM     Status:  MEDLINE    
Systemic administration of microorganisms into tumor-bearing mice revealed preferential accumulation in tumors in comparison to clearance in organs such as spleen and liver. Here we compared the efficiency of tumor-specific colonization of pathogenic Salmonella typhimurium strains 14028 and SL1344 to the enteroinvasive Escherichia coli 4608-58 strain and to the attenuated Salmonella flexneri 2a SC602 strain, as well as to the uropathogenic E. coli CFT073, the non-pathogenic E. coli Top10, and the probiotic E. coli Nissle 1917 strain. All strains colonized and replicated in tumors efficiently each resulting in more than 1 x 10(8) colony-forming units per gram tumor tissue. Colonization of spleen and liver were significantly lower when E. coli strains were used in comparison to S. typhimurium and the non-pathogenic strains did not colonize those organs at all. Further investigation of E. coli Nissle 1917 showed that no drastic differences in colonization and amplification were seen when immunocompetent and immunocompromised animals were used, and we were able to show that E. coli Nissle 1917 replicates at the border of live and necrotic tumor tissue. We also demonstrated exogenously applied L-arabinose-dependent gene activation in colonized tumors in live mice. These findings will prepare the way for bacterium-mediated controlled protein delivery to solid tumors.
Jochen Stritzker; Stephanie Weibel; Philip J Hill; Tobias A Oelschlaeger; Werner Goebel; Aladar A Szalay
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-19
Journal Detail:
Title:  International journal of medical microbiology : IJMM     Volume:  297     ISSN:  1438-4221     ISO Abbreviation:  Int. J. Med. Microbiol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-08     Completed Date:  2007-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100898849     Medline TA:  Int J Med Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  151-62     Citation Subset:  IM    
Genelux Corporation, 3030 Bunker Hill St., Ste. 310, San Diego, CA 92109, USA.
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MeSH Terms
Arabinose / biosynthesis,  genetics
Breast Neoplasms / microbiology,  therapy*
Carcinoma, Intraductal, Noninfiltrating / microbiology,  therapy*
Colony Count, Microbial
Escherichia coli* / growth & development,  metabolism
Gene Expression*
Genetic Vectors / physiology
Liver / microbiology
Mammary Neoplasms, Experimental / microbiology,  therapy*
Mice, Inbred BALB C
Mice, Nude
Recombinant Proteins / biosynthesis
Salmonella typhimurium / growth & development
Shigella flexneri / growth & development
Species Specificity
Spleen / microbiology
Reg. No./Substance:
0/Recombinant Proteins; 147-81-9/Arabinose

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