Document Detail

Tumor necrosis factor mediates the protective effect of Freund's adjuvant against autoimmune diabetes in BB rats.
MedLine Citation:
PMID:  7575997     Owner:  NLM     Status:  MEDLINE    
In previous studies we reported that a single injection of complete Freund's adjuvant (CFA) in early life inhibits the development of autoimmune diabetes in BB diabetes-prone (DP) rats, and that CFA upregulates tumor necrosis factor-alpha (TNF alpha) production by macrophages of DP rats. In this study, we asked if CFA-induced prevention of diabetes in DP rats might be mediated by TNF alpha. Chronic intraperitoneal injection of TNF alpha, 20 micrograms daily from age 50 to 110 days, significantly decreased diabetes incidence in DP rats from 83% (15 of 18) to 37% (seven of 19) at age 110 days. Similarly, a single intraperitoneal injection of CFA, 100 microliters at age 26 days, significantly decreased diabetes incidence from 80% (16 of 20) to 50% (10 of 20) at age 110 days. Prevention by either TNF alpha or CFA was associated with decreased insulitis and preservation of islet beta-cells. By contrast, treatment of DP rats with CFA, plus antiserum to TNF alpha three times a week from age 26 to 110 days, obviated CFA-induced protection against beta-cell destructive insulitis and diabetes. Thus, diabetes incidence was 75% (18 of 24) in DP rats treated with CFA plus anti-TNF alpha antiserum, similar to that in untreated DP rats (80%), but only 44% (eight of 18) in DP rats treated with CFA plus control serum, similar to that in DP rats with CFA alone (50%). These results suggest that TNF alpha may be a mediator of the protective effects of CFA against autoimmune diabetes development in BB rats.
A Rabinovitch; W L Suarez-Pinzon; P H Lapchak; A Meager; R F Power
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of autoimmunity     Volume:  8     ISSN:  0896-8411     ISO Abbreviation:  J. Autoimmun.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-12-12     Completed Date:  1995-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8812164     Medline TA:  J Autoimmun     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  357-66     Citation Subset:  IM    
Department of Medicine, University of Alberta, Edmonton, Canada.
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MeSH Terms
Autoimmunity / drug effects*
Diabetes Mellitus, Type 1 / prevention & control*
Freund's Adjuvant / administration & dosage,  therapeutic use*
Immune Sera / pharmacology
Injections, Intraperitoneal
Islets of Langerhans / pathology
Rats, Inbred BB
Tumor Necrosis Factor-alpha / immunology,  therapeutic use*
Reg. No./Substance:
0/Immune Sera; 0/Tumor Necrosis Factor-alpha; 9007-81-2/Freund's Adjuvant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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