Document Detail


Tumor necrosis factor induces GSK3 kinase-mediated cross-tolerance to endotoxin in macrophages.
MedLine Citation:
PMID:  21602809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endotoxin tolerance, a key mechanism for suppressing excessive inflammatory cytokine production, is induced by prior exposure of macrophages to Toll-like receptor (TLR) ligands. Induction of cross-tolerance to endotoxin by endogenous cytokines has not been investigated. Here we show that prior exposure to tumor necrosis factor (TNF) induced a tolerant state in macrophages, with less cytokine production after challenge with lipopolysaccharide (LPS) and protection from LPS-induced death. TNF-induced cross-tolerization was mediated by suppression of LPS-induced signaling and chromatin remodeling. TNF-induced cross-tolerance was dependent on the kinase GSK3, which suppressed chromatin accessibility and promoted rapid termination of signaling via the transcription factor NF-κB by augmenting negative feedback by the signaling inhibitors A20 and IκBα. Our results demonstrate an unexpected homeostatic function for TNF and a GSK3-mediated mechanism for the prevention of prolonged and excessive inflammation.
Authors:
Sung Ho Park; Kyung-Hyun Park-Min; Janice Chen; Xiaoyu Hu; Lionel B Ivashkiv
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-05-22
Journal Detail:
Title:  Nature immunology     Volume:  12     ISSN:  1529-2916     ISO Abbreviation:  Nat. Immunol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-20     Completed Date:  2011-08-23     Revised Date:  2012-05-10    
Medline Journal Info:
Nlm Unique ID:  100941354     Medline TA:  Nat Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  607-15     Citation Subset:  IM    
Affiliation:
Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, New York, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatin Assembly and Disassembly / drug effects,  immunology
Cysteine Endopeptidases / immunology,  metabolism
Cytokines / biosynthesis,  immunology
Endotoxins / immunology*
Female
Glycogen Synthase Kinase 3 / immunology*
I-kappa B Proteins / immunology,  metabolism
Intracellular Signaling Peptides and Proteins / immunology,  metabolism
Lipopolysaccharides / immunology
Macrophages / drug effects,  immunology*
Male
Mice
Mice, Inbred C57BL
NF-kappa B / immunology,  metabolism
Toll-Like Receptors / immunology,  metabolism
Tumor Necrosis Factor-alpha / immunology*
Grant Support
ID/Acronym/Agency:
R01 AI046712-11/AI/NIAID NIH HHS; R01 AI046712-12/AI/NIAID NIH HHS; R01 AI046712-13/AI/NIAID NIH HHS; R01 AR046713-11A1/AR/NIAMS NIH HHS; R01 AR046713-12/AR/NIAMS NIH HHS; R01 AR046713-13/AR/NIAMS NIH HHS; R01 AR050401/AR/NIAMS NIH HHS; R01 AR050401-06A2/AR/NIAMS NIH HHS; R01 AR050401-07/AR/NIAMS NIH HHS; R01 AR056729-03/AR/NIAMS NIH HHS; R01 DE019420-03/DE/NIDCR NIH HHS; R01 DE019420-04/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/Endotoxins; 0/I-kappa B Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Lipopolysaccharides; 0/NF-kappa B; 0/Toll-Like Receptors; 0/Tumor Necrosis Factor-alpha; 139874-52-5/NF-kappaB inhibitor alpha; EC 2.7.11.26/Glycogen Synthase Kinase 3; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.-/Tnfaip3 protein, mouse

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