Document Detail


Tumor necrosis factor-alpha and interleukin-18 modulate neuronal cell fate in embryonic neural progenitor culture.
MedLine Citation:
PMID:  16054598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neural progenitor cells (NPCs) in developing and adult CNS are capable of giving rise to various neuronal and glial cell populations. Neurogenesis in the adult hippocampus has been found to be inhibited by a proinflammatory cytokine, interleukin-6 (IL-6), suggesting that activated microglia in the inflamed brain may control neurogenesis. Yet, little is known about the effect of microglia-derived factors on the cell fate of embryonic NPCs. In this study, we show that neurons with betaIII-tubulin immunoreactivity in the NPC culture were reduced by the condition media collected from microglia treated with endotoxin lipopolysaccharide (LPS/M-CM). Treatment with pentoxifylline (PTX), an inhibitor for tumor necrosis factor-alpha (TNF-alpha) secretion from LPS-activated microglia, blocked the reduction of betaIII-tubulin+ cells in NPC culture. Furthermore, treatment of NPCs with interleukin-18 (IL-18), a recently discovered proinflammatory cytokine, also decreased the number of betaIII-tubulin+ cells in a dose- and time-dependent manner. Surprisingly, we also observed that the remaining betaIII-tubulin+ cells in the LPS/M-CM-treated culture exhibited more branching neurites. Thus, the activated microglia-derived cytokines, TNF-alpha and IL-18, may either inhibit the neuronal differentiation or induce neuronal cell death in the NPC culture, whereas these cells may also produce factors to improve the neurite branching in the NPC culture.
Authors:
Yu-Peng Liu; Hsin-I Lin; Shun-Fen Tzeng
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  1054     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-09-02     Completed Date:  2005-12-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  152-8     Citation Subset:  IM    
Affiliation:
Department of Life Sciences, National Cheng Kung University, #1 Ta-Hsueh Rd., Tainan 70101, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Blotting, Western / methods
Cell Count / methods
Cell Death / drug effects,  physiology
Cell Differentiation / drug effects,  physiology
Cells, Cultured
Dose-Response Relationship, Drug
Embryo, Mammalian
Immunohistochemistry / methods
Interleukin-18 / metabolism*
Lipopolysaccharides / pharmacology
Microglia / drug effects,  physiology
Neurons / drug effects,  physiology*
Pertussis Toxin / pharmacology
Rats
Rats, Sprague-Dawley
Stem Cells / drug effects,  physiology*
Tubulin / metabolism
Tumor Necrosis Factor-alpha / metabolism*
Chemical
Reg. No./Substance:
0/Interleukin-18; 0/Lipopolysaccharides; 0/Tubulin; 0/Tumor Necrosis Factor-alpha; 0/beta III-tubulin protein, rat; EC 2.4.2.31/Pertussis Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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