Document Detail


Tumor necrosis factor alpha-induced vascular leakage involves PECAM1 phosphorylation.
MedLine Citation:
PMID:  8764109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Herein we show that exposure of human umbilical vein endothelial cells to tumor necrosis factor alpha (TNFalpha) led to platelet endothelial cell adhesion molecule-1 (PECAM1) surface redistribution, disruption of cytoskeleton connections, and increased PECAM1 phosphorylation, accompanied by increased permeability to macromolecules. The in vitro use of inhibitors of tyrosine or serine-threonine kinases could prevent both PECAM1 surface redistribution and the increase in permeability induced by the cytokine. In vivo administration of lavendustin A, a natural tyrosine kinase inhibitor, protected endothelial cells from TNFalpha-dependent vascular leakage in mouse liver. We propose that the involvement of PECAM1 in TNFalpha-mediated effects on vascular permeability may depend on a dynamically regulated cytoskeletal association, related to the degree of PECAM1 phosphorylation.
Authors:
E Ferrero; A Villa; M E Ferrero; E Toninelli; J R Bender; R Pardi; M R Zocchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  56     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-09-20     Completed Date:  1996-09-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3211-5     Citation Subset:  IM    
Affiliation:
Laboratory of Tumor Immunology, Scientific Institute San Raffaele, Italy.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / pharmacology
Antigens, CD31
Antigens, Differentiation, Myelomonocytic / metabolism*
Benzoquinones
Capillary Permeability / drug effects*
Cell Adhesion Molecules / metabolism*
Cell Compartmentation
Cells, Cultured
Endothelium, Vascular / metabolism*
Enzyme Inhibitors / pharmacology
Fluorescent Antibody Technique, Indirect
Gene Expression
Humans
Lactams, Macrocyclic
Phosphorylation
Protein Kinase Inhibitors
Quinones / pharmacology
RNA, Messenger / genetics
Staurosporine
Tumor Necrosis Factor-alpha / pharmacology*
Umbilical Veins
Grant Support
ID/Acronym/Agency:
HL43331/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Antigens, CD31; 0/Antigens, Differentiation, Myelomonocytic; 0/Benzoquinones; 0/Cell Adhesion Molecules; 0/Enzyme Inhibitors; 0/Lactams, Macrocyclic; 0/Protein Kinase Inhibitors; 0/Quinones; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 62996-74-1/Staurosporine; 70563-58-5/herbimycin

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