| Tumor necrosis factor activity of pancreatic islets. | |
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MedLine Citation:
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PMID: 9277398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tumor necrosis factor (TNF) is involved in the pathogenesis of acute sepsis-induced organ injury and has been implicated as a mediator of metabolic alterations observed during sepsis. Pancreatic islet cell function may be significantly compromised during sepsis or endotoxemia, and sepsis also increases plasma levels of epinephrine, a modifier of islet insulin secretion. We proposed that islets exposed to bacterial lipopolysaccharide (LPS) produce TNF and that epinephrine attenuates islet secretory activity. We monitored the effects of LPS and epinephrine on TNF and insulin activity of isolated Wistar-Furth rat islets (pancreas digested with collagenase, islets isolated using Ficoll gradients; n = 4 islet populations, each with 632 +/- 11 islets/2.5 ml culture medium). Islets were incubated (37 degrees C, 5% CO2) 3 days. LPS (Escherichia coli, 1 microgram/ml) and epinephrine (14 micrograms/ml) were added to the islets, and incubations were continued for 1-4 h. Glucose (Beckman Glucose Analyzer), insulin (radioimmunoassay), and TNF (L929 cytotoxicity assay) were measured in the islet medium samples at 1- to 4-h time points. In the conditioned medium, glucose decreased (P < 0.05), insulin increased (P < 0.05), and exposure to LPS did not alter these levels [P = not significant (NS)] but did increase TNF activity by 400% (P < 0.05). Epinephrine reduced insulin by 38-43% (P < 0.05) and TNF by 20-25% (P < 0.05) but had no effect on glucose levels (P = NS). We conclude that insulin is secreted from isolated islets and that exposure to LPS acutely increases islet-derived TNF activity, whereas epinephrine modifies TNF and insulin secretion of rat pancreatic islets. |
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Authors:
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S K Leeper-Woodford; B W Tobin |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 273 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 1997-09-24 Completed Date: 1997-09-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E433-7 Citation Subset: IM |
Affiliation:
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Department of Physiology, Mercer University School of Medicine, Macon, Georgia 31207, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Culture Media / metabolism Epinephrine / pharmacology Glucose / metabolism Insulin / metabolism Islets of Langerhans / drug effects, metabolism* Lipopolysaccharides / pharmacology Male Rats Rats, Inbred WF Tumor Necrosis Factor-alpha / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HL-52917/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 11061-68-0/Insulin; 50-99-7/Glucose; 51-43-4/Epinephrine |
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