Document Detail

Tumor cell/endothelial cell tight contact upregulates endothelial adhesion molecule expression mediated by NFkappaB: differential role of the shear stress.
MedLine Citation:
PMID:  19944683     Owner:  NLM     Status:  MEDLINE    
Cancer metastasis is a multistep process involving cell-cell interactions, but little is known about the adhesive interactions and signaling events during extravasation of tumor cells (TCs). In this study, cell adhesion molecule (CAM) expression was investigated using an in vitro assay, in which TCs were seeded onto an endothelial cell (ECs) monolayer and cocultured during 5 h. Flow cytometry, confocal microscopy as well as western blot analysis indicated that endothelial ICAM-1 (Inter Cellular Adhesion Molecule-1), VCAM-1 (Vascular Adhesion Molecule-1) and E-selectin were up-regulated after TC-EC coculture, whereas no change was observed for CAMs expression in tumor cells. This increased CAMs expression required tight contact between TCs and ECs. Incubation of ECs with the pyrrolidine-dithiocarbamate NFkappaB inhibitor prior to coculture, fully prevented coculture-induced expression of endothelial CAMs. Using specific blocking antibodies we showed an implication of ICAM-1 and VCAM-1 for TCs extravasation and VCAM-1 for adhesion. Moreover, fluid flow experiments revealed that high shear stress totally abolished coculture-induced as well as TNFalpha-induced CAMs over-expression. This study suggests that TCs could act as a potent inflammatory stimulus on ECs by inducing CAMs expression via NFkappaB activation, and that this action can be modulated by shear stress.
Oualid Haddad; Roxana Chotard-Ghodsnia; Claude Verdier; Alain Duperray
Related Documents :
10374723 - Mechanics of leukocyte deformation and adhesion to endothelium in shear flow.
15797243 - Vascular damage by unstable hemoglobins: the role of heme-depleted globin.
18028133 - Immobilized gradients of epidermal growth factor promote accelerated and directed kerat...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-26
Journal Detail:
Title:  Experimental cell research     Volume:  316     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  615-26     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
INSERM U823, Grenoble, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Western
Cell Communication*
Cell Line, Tumor
Cells, Cultured
Coculture Techniques
E-Selectin / metabolism
Endothelial Cells / metabolism*
Flow Cytometry
Intercellular Adhesion Molecule-1 / metabolism*
NF-kappa B / pharmacology*
Signal Transduction
Stress, Mechanical*
Up-Regulation / drug effects*
Vascular Cell Adhesion Molecule-1 / metabolism
Reg. No./Substance:
0/E-Selectin; 0/NF-kappa B; 0/Vascular Cell Adhesion Molecule-1; 126547-89-5/Intercellular Adhesion Molecule-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Analysis of local immunity in squamous cell carcinoma of the tongue and lower lip.
Next Document:  The multiple functions of Numb.