Document Detail


Tumor cell invasion assays.
MedLine Citation:
PMID:  15576908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of tumor cells to invade is one of the hallmarks of the metastatic phenotype. To elucidate the mechanisms by which tumor cells acquire an invasive phenotype, in vitro assays have been developed that mimic the in vivo process. The most commonly used in vitro invasion assay is a modified Boyden chamber assay using a basement membrane matrix preparation, Matrigel, as the matrix barrier and NIH-3T3 conditioned media as the chemoattractant. The results obtained using this assay show a strong correlation between the ability of tumor cells to invade in vitro and their invasive behavior in vivo, which validates this assay as a measure of invasive potential. The methods presented in this chapter outline how the Matrigel in vitro invasion assay is performed.
Authors:
Leslie M Shaw
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  294     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-03     Completed Date:  2005-04-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-105     Citation Subset:  IM    
Affiliation:
Division of Cancer Biology and Angiogenesis, Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cell Culture Techniques / methods
Cell Movement / physiology
Collagen
Culture Media, Conditioned
Drug Combinations
Extracellular Matrix / physiology
Laminin
Mice
Neoplasm Invasiveness / pathology*
Proteoglycans
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Drug Combinations; 0/Laminin; 0/Proteoglycans; 119978-18-6/matrigel; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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