Document Detail


Tumor Necrosis Factor-alpha- and interleukin-1-induced cellular responses: coupling proteomic and genomic information.
MedLine Citation:
PMID:  17503796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pro-inflammatory cytokines, Tumor Necrosis Factor-alpha (TNFalpha) and Interleukin-1 (IL-1) mediate the innate immune response. Dysregulation of the innate immune response contributes to the pathogenesis of cancer, arthritis, and congestive heart failure. TNFalpha- and IL-1-induced changes in gene expression are mediated by similar transcription factors; however, TNFalpha and IL-1 receptor knock-out mice differ in their sensitivities to a known initiator (lipopolysaccharide, LPS) of the innate immune response. The contrasting responses to LPS indicate that TNFalpha and IL-1 regulate different processes. A large-scale proteomic analysis of TNFalpha- and IL-1-induced responses was undertaken to identify processes uniquely regulated by TNFalpha and IL-1. When combined with genomic studies, our results indicate that TNFalpha, but not IL-1, mediates cell cycle arrest.
Authors:
Lee W Ott; Katheryn A Resing; Alecia W Sizemore; Joshua W Heyen; Ross R Cocklin; Nathan M Pedrick; H Cary Woods; Jake Y Chen; Mark G Goebl; Frank A Witzmann; Maureen A Harrington
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-05-16
Journal Detail:
Title:  Journal of proteome research     Volume:  6     ISSN:  1535-3893     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-01     Completed Date:  2007-07-18     Revised Date:  2011-09-13    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2176-85     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, MS 4053, Indianapolis, Indiana 46202, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / drug effects
Cell Line
Cell Membrane Permeability / drug effects
Cytoplasm / drug effects,  genetics,  metabolism
Genomics*
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / genetics,  metabolism
Interleukin-1 / pharmacology*
Mitochondrial Membranes / drug effects
Proteins / analysis,  genetics
Proteomics*
RNA, Messenger / analysis
Transcription, Genetic / drug effects
Tumor Necrosis Factor-alpha / pharmacology*
Grant Support
ID/Acronym/Agency:
R21 AA015698-01/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/Interleukin-1; 0/Proteins; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha
Comments/Corrections

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