Document Detail


Tumor cell growth inhibition and cell differentiation analysis in a canine mammary tumor cell line (MCM-B2) treated with four chemical reagents.
MedLine Citation:
PMID:  19959889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we examined the effects of four chemical reagents, 5 azacytidine (5azaC), all-trans-retinoic acid (ATRA), Phorbol 12-myristate 13-acetate (TPA) and trichostatin A (TSA), on an MCM-B2 canine mammary tumor cell line. Growth of the MCM-B2 cells was inhibited by addition of any of these four chemical reagents in a dose-dependent manner. TPA-treated cells became blast-cell-like and had high expressions of cyclin D, cyclin B and PCNA. Both ATRA-treated and 5azaC-treated cells showed decreased expression of cell cycle related molecules and increased expressions of the mammary epithelial marker cytokeratin 18 and underwent morphological changes. ATRA-treated cells were converted from the originally spindle-shaped cells into cubic-shaped cells, and 5azaC-treated cells became fibroblast-like. In the TSA-treated cells, the cytoplasm was enlarged, increasing the cytoplasm/nucleus ratio, and the expressions of cell cycle-promoting molecules (cyclin D and PCNA), as well as cell cycle-inhibiting factors (p21(WAF1) and p27(kip1)), were increased. These results demonstrated the growth-inhibiting, differentiation-inducing and antitumor effects of each of these four chemicals on the unique phenotype of canine mammary tumors.
Authors:
Manabu WATANABE; Iris Yun-Ju CHEN; Yasuhiro ISHIKURA; Takayuki NAKAGAWA; Manabu MOCHIZUKI; Ryohei NISHIMURA; Nobuo SASAKI; Sumio SUGANO
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  71     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-12-04     Completed Date:  2010-01-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1413-7     Citation Subset:  IM    
Affiliation:
Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Minato-ku, Tokyo, Japan. wata@ims.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology*
Cell Differentiation / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Dog Diseases / drug therapy
Dogs
Female
Mammary Neoplasms, Animal / drug therapy*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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