| Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice. | |
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MedLine Citation:
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PMID: 17141210 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The germ-line cells of many animals possess a characteristic cytoplasmic structure termed nuage or germinal granules. In mice, nuage that is prominent in postnatal male germ cells is also called intermitochondrial cement or chromatoid bodies. TDRD1/MTR-1, which contains Tudor domain repeats, is a specific component of the mouse nuage, analogously to Drosophila Tudor, a constituent of polar granules/nuage in oocytes and embryos. We show that TDRD6 and TDRD7/TRAP, which also contain multiple Tudor domains, specifically localize to nuage and form a ribonucleoprotein complex together with TDRD1/MTR-1. The characteristic co-localization of TDRD1, 6 and 7 was disrupted in a mutant of mouse vasa homologue/DEAD box polypeptide 4 (Mvh/Ddx4), which encodes another evolutionarily conserved component of nuage. In vivo over-expression experiments of the TDRD proteins and truncated forms during male germ cell differentiation showed that a single Tudor domain is a structural unit that localizes or accumulates to nuage, but the expression of the truncated, putative dominant negative forms is detrimental to meiotic spermatocytes. These results indicate that the Tudor-related proteins, which contain multiple repeats of the Tudor domain, constitute an evolutionarily conserved class of nuage components in the germ-line, and their localization or accumulation to nuage is likely conferred by a Tudor domain structure and downstream of Mvh, while the characteristic repeated architecture of the domain is functionally essential for the differentiation of germ cells. |
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Authors:
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Mihoko Hosokawa; Masanobu Shoji; Kouichi Kitamura; Takashi Tanaka; Toshiaki Noce; Shinichiro Chuma; Norio Nakatsuji |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-06 |
Journal Detail:
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Title: Developmental biology Volume: 301 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-25 Completed Date: 2007-03-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 38-52 Citation Subset: IM |
Affiliation:
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Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Kyoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence DNA Primers Fluorescent Antibody Technique Male Mice Mice, Inbred ICR Microscopy, Immunoelectron Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction Ribonucleoproteins / chemistry, metabolism, physiology* Ribonucleoproteins, Small Nuclear / chemistry, metabolism, physiology* Sequence Homology, Amino Acid Spermatozoa / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Ribonucleoproteins; 0/Ribonucleoproteins, Small Nuclear; 0/TDRD6 protein, mouse; 0/TDRD7 protein, mouse; 0/Tdrd1 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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