Document Detail


Tubulointerstitial nephritis associated with a novel mitochondrial point mutation.
MedLine Citation:
PMID:  11231339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Nephropathy caused by mitochondrial disorders is a relatively newly recognized disease. Only a few cases have been reported in the literature, and most of them are proximal tubulopathy-presenting Fanconi syndrome. Here we report on a novel mutation in two familial cases of tubulointerstitial nephropathy associated with concentrating defect. METHODS: Renal biopsy specimens were examined by light microscopy and electron microscopy. Mitochondrial genomic DNA isolated from renal biopsy specimens was amplified by polymerase chain reaction (PCR) and sequenced in its entirety. The DNA sequences were analyzed by (1) comparing with the Anderson et al's mitochondrial sequences; (2) comparing with DNA sequences obtained from 97 human controls, including both healthy individuals and patients with renal diseases; and (3) comparing with the counterparts in 90 different species. RESULTS: Dismorphic mitochondria with occasional intramitochondrial inclusions were found in the renal tubular epithelial cells. A novel mitochondrial point mutation was identified at the position 608, that is, the distal end of the anticodon stem of the tRNA(Phe) molecule. The A to G substitution at this position was not observed in 97 human controls and was found to be highly conserved in evolution. CONCLUSIONS: We have identified an A608G mutation of mitochondrial genome in two cases whose presentation include tubulointerstitial nephritis and stroke.
Authors:
C Y Tzen; J D Tsai; T Y Wu; B F Chen; M L Chen; S P Lin; S C Chen
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney international     Volume:  59     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  846-54     Citation Subset:  IM    
Affiliation:
Department of Pathology, Mackay Memorial Hospital, Taipei, Taiwan. jeffrey@ms2.mmh.org.tw
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence / genetics
Child, Preschool
Conserved Sequence / genetics
DNA, Mitochondrial / metabolism
Evolution
Humans
Kidney / metabolism,  pathology
Male
Microscopy, Electron
Mitochondria / genetics*
Molecular Sequence Data
Nephritis, Interstitial / genetics*,  pathology
Point Mutation*
RNA, Transfer, Phe / genetics
Reference Values
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial; 0/RNA, Transfer, Phe

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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