| Tubular overexpression of transforming growth factor-beta1 induces autophagy and fibrosis but not mesenchymal transition of renal epithelial cells. | |
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MedLine Citation:
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PMID: 20616344 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently showed in a tetracycline-controlled transgenic mouse model that overexpression of transforming growth factor (TGF)-beta1 in renal tubules induces widespread peritubular fibrosis and focal degeneration of nephrons. In the present study we have analyzed the mechanisms underlying these phenomena. The initial response to tubular cell-derived TGF-beta1 consisted of a robust proliferation of peritubular cells and deposition of collagen. On sustained expression, nephrons degenerated in a focal pattern. This process started with tubular dedifferentiation and proceeded to total decomposition of tubular cells by autophagy. The final outcome was empty collapsed remnants of tubular basement membrane embedded into a dense collagenous fibrous tissue. The corresponding glomeruli survived as atubular remnants. Thus, TGF-beta1 driven autophagy may represent a novel mechanism of tubular decomposition. The fibrosis seen in between intact tubules and in areas of tubular decomposition resulted from myofibroblasts that were derived from local fibroblasts. No evidence was found for a transition of tubular cells into myofibroblasts. Neither tracing of injured tubules in electron micrographs nor genetic tagging of tubular epithelial cells revealed cells transgressing the tubular basement membrane. In conclusion, overexpression of TGF-beta1 in renal tubules in vivo induces interstitial proliferation, tubular autophagy, and fibrosis, but not epithelial-to-mesenchymal transition. |
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Authors:
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Robert Koesters; Brigitte Kaissling; Michel Lehir; Nicolas Picard; Franziska Theilig; Rolf Gebhardt; Adam B Glick; Brunhilde Hähnel; Hiltraud Hosser; Hermann-Josef Gröne; Wilhelm Kriz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-08 |
Journal Detail:
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Title: The American journal of pathology Volume: 177 ISSN: 1525-2191 ISO Abbreviation: Am. J. Pathol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2011-01-25 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 632-43 Citation Subset: AIM; IM |
Affiliation:
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Institute of Human Genetics, University of Heidelberg, Heidelberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autophagy / physiology* Epithelial Cells / physiology*, ultrastructure Epithelial-Mesenchymal Transition / physiology* Fibrosis / metabolism, pathology Kidney* / cytology, metabolism, pathology Kidney Tubules* / metabolism, ultrastructure Mice Mice, Inbred Strains Mice, Transgenic Transforming Growth Factor beta1 / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Transforming Growth Factor beta1 |
| Comments/Corrections | |
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