| Tubers from potato lines expressing a tomato Kunitz protease inhibitor are substantially equivalent to parental and transgenic controls. | |
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MedLine Citation:
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PMID: 20051032 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recombinant protease inhibitors represent useful tools for the development of insect-resistant transgenic crops, but questions have been raised in recent years about the impact of these proteins on endogenous proteases and chemical composition of derived food products. In this study, we performed a detailed compositional analysis of tubers from potato lines expressing the broad-spectrum inhibitor of Ser and Asp proteases, tomato cathepsin D inhibitor (SlCDI), to detect possible unintended effects on tuber composition. A compositional analysis of key nutrients and toxic chemicals was carried out with tubers of SlCDI-expressing and control (comparator) lines, followed by a two-dimensional gel electrophoresis (2-DE) proteomic profiling of total and allergenic proteins to detect eventual effects at the proteome level. No significant differences were observed among control and SlCDI-expressing lines for most chemicals assayed, in line with the very low abundance of SlCDI in tubers. Likewise, proteins detected after 2-DE showed no quantitative variation among the lines, except for a few proteins in some control and test lines, independent of slcdi transgene expression. Components of the patatin storage protein complex and Kunitz protease inhibitors immunodetected after 2-DE showed unaltered deposition patterns in SlCDI-expressing lines, clearly suggesting a null impact of slcdi on the intrinsic allergenic potential of potato tubers. These data suggest, overall, a null impact of slcdi expression on tuber composition and substantial equivalence between comparator and SlCDI-expressing tubers despite reported effects on leaf protein catabolism. They also illustrate the usefulness of proteomics as a tool to assess the authenticity of foods derived from novel-generation transgenic plants. |
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Authors:
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Moustafa Khalf; Charles Goulet; Juan Vorster; France Brunelle; Rapha?l Anguenot; Isma?l Fliss; Dominique Michaud |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-28 |
Journal Detail:
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Title: Plant biotechnology journal Volume: 8 ISSN: 1467-7652 ISO Abbreviation: Plant Biotechnol. J. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-18 Completed Date: 2010-04-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101201889 Medline TA: Plant Biotechnol J Country: England |
Other Details:
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Languages: eng Pagination: 155-69 Citation Subset: IM |
Affiliation:
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CRH/INAF, Pavillon des Services (INAF), Universit? Laval, Qu?bec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Electrophoresis, Gel, Two-Dimensional Lycopersicon esculentum / genetics* Models, Molecular Peptides / genetics*, metabolism Plant Proteins / genetics*, metabolism Plant Tubers / genetics, metabolism* Plants, Genetically Modified / genetics, metabolism Protein Structure, Tertiary Proteome / metabolism Solanum tuberosum / genetics, metabolism* Transgenes |
| Chemical | |
Reg. No./Substance:
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0/Kunitz-type protease inhibitor, plant; 0/Peptides; 0/Plant Proteins; 0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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