| Tryptophan-transducing bacteriophages: in vitro studies with restriction endonucleases HindII + III and Escherichia coli ribonucleic acid polymerase. | |
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MedLine Citation:
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PMID: 334724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The HindII + III restriction enzyme fragmentation pattern of various lambda-phi80trp deoxyribonucleic acid molecules is presented. An analysis of deoxyribonucleic acid molecules carrying deletions ending within the trp regulatory elements and a deoxyribonucleic acid molecule carrying a deletion within trpE indicates that a fragment of 8.3 X 10(5) daltons contains at least part of the trp promoter, the entire trp leader region, and part of the trpE gene. The observation that ribonucleic acid polymerase, when present in the HindII + III digestion mixture, results in the fusion of this 8.3 X 10(5)-dalton fragment to the preceding bacterial fragment suggests that HindII + III cuts within trpP. |
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Authors:
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B B Jones; W S Reznikoff |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 132 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1977 Oct |
Date Detail:
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Created Date: 1977-12-29 Completed Date: 1977-12-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 270-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Coliphages
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analysis,
genetics*,
metabolism DNA Restriction Enzymes / metabolism DNA, Viral / genetics*, metabolism DNA-Directed RNA Polymerases / metabolism Escherichia coli / enzymology Molecular Weight Operon* Protein Binding Transduction, Genetic* Tryptophan / biosynthesis, genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 73-22-3/Tryptophan; EC 2.7.7.6/DNA-Directed RNA Polymerases; EC 3.1.21.-/DNA Restriction Enzymes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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