Document Detail


Tryptophan-dependent indole-3-acetic acid biosynthesis by 'IAA-synthase' proceeds via indole-3-acetamide.
MedLine Citation:
PMID:  19268331     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants are suggested to produce their major growth promoting phytohormone, indole-3-acetic acid (IAA), via multiple redundantly operating pathways. Although great effort has been made and plenty of possible routes have been proposed based on experimental evidence, a complete pathway for IAA production has yet to be demonstrated. In this study, an in-vitro approach was taken to examine the conversion of l-tryptophan (l-trp) to IAA by gas chromatography-mass spectrometry (GC-MS). Especially the influence of putative reaction intermediates on the enzymatic conversion of l-trp to IAA was analyzed. Among the substances tested only indole-3-acetamide (IAM) showed a pronounced effect on the l-trp conversion. We additionally report that IAM is synthesized from l-trp and that it is further converted to IAA by the utilized cell free Arabidopsis extract. Together, our results underscore the functionality of an IAM-dependent auxin biosynthesis pathway in Arabidopsis thaliana.
Authors:
Stephan Pollmann; Petra Düchting; Elmar W Weiler
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-04
Journal Detail:
Title:  Phytochemistry     Volume:  70     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-24     Completed Date:  2009-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  523-31     Citation Subset:  IM    
Affiliation:
Department of Plant Physiology, Ruhr-University Bochum, Universitätsstrasse 150, 44801 Bochum, Germany. Stephan.Pollmann@rub.de
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / physiology
Aminohydrolases / genetics
Arabidopsis / drug effects,  genetics,  metabolism*
Gas Chromatography-Mass Spectrometry
Indoleacetic Acids / metabolism*,  pharmacology
Tryptamines / pharmacology
Tryptophan / metabolism*
Chemical
Reg. No./Substance:
0/Indoleacetic Acids; 0/Tryptamines; 61-54-1/tryptamine; 73-22-3/Tryptophan; 87-51-4/indoleacetic acid; 879-37-8/indoleacetamide; EC 3.5.-/Amidohydrolases; EC 3.5.1.4/amidase; EC 3.5.4.-/Aminohydrolases; EC 3.5.5.1/nitrilase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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