| Tryptophan-dependent indole-3-acetic acid biosynthesis by 'IAA-synthase' proceeds via indole-3-acetamide. | |
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MedLine Citation:
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PMID: 19268331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plants are suggested to produce their major growth promoting phytohormone, indole-3-acetic acid (IAA), via multiple redundantly operating pathways. Although great effort has been made and plenty of possible routes have been proposed based on experimental evidence, a complete pathway for IAA production has yet to be demonstrated. In this study, an in-vitro approach was taken to examine the conversion of l-tryptophan (l-trp) to IAA by gas chromatography-mass spectrometry (GC-MS). Especially the influence of putative reaction intermediates on the enzymatic conversion of l-trp to IAA was analyzed. Among the substances tested only indole-3-acetamide (IAM) showed a pronounced effect on the l-trp conversion. We additionally report that IAM is synthesized from l-trp and that it is further converted to IAA by the utilized cell free Arabidopsis extract. Together, our results underscore the functionality of an IAM-dependent auxin biosynthesis pathway in Arabidopsis thaliana. |
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Authors:
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Stephan Pollmann; Petra Düchting; Elmar W Weiler |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-04 |
Journal Detail:
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Title: Phytochemistry Volume: 70 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-24 Completed Date: 2009-07-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 523-31 Citation Subset: IM |
Affiliation:
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Department of Plant Physiology, Ruhr-University Bochum, Universitätsstrasse 150, 44801 Bochum, Germany. Stephan.Pollmann@rub.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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physiology Aminohydrolases / genetics Arabidopsis / drug effects, genetics, metabolism* Gas Chromatography-Mass Spectrometry Indoleacetic Acids / metabolism*, pharmacology Tryptamines / pharmacology Tryptophan / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Indoleacetic Acids; 0/Tryptamines; 61-54-1/tryptamine; 73-22-3/Tryptophan; 87-51-4/indoleacetic acid; 879-37-8/indoleacetamide; EC 3.5.-/Amidohydrolases; EC 3.5.1.4/amidase; EC 3.5.4.-/Aminohydrolases; EC 3.5.5.1/nitrilase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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