| Tryptophan degradation in patients with gynecological cancer correlates with immune activation. | |
| | |
MedLine Citation:
|
PMID: 15896467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Tryptophan degradation by the enzyme indoleamine-(2,3)-dioxy genase (IDO) and neopterin production are induced within cellular immune activation by stimulation of monocyte-derived macrophages and dendritic cells with cytokine interferon-gamma. Deprivation of tryptophan represents an important antimicrobial and antitumoral immune defence mechanism but it also suppresses T-cell proliferation. Recently tryptophan degradation by tumor cells was proposed as strategy to escape immune response. In this study the relationship between tryptophan degradation and immune activation was examined in 20 patients with gynecological cancer. Concentrations of tryptophan and kynurenine were measured by HPLC in sera of patients, and to estimate IDO activity, the kynurenine to tryptophan ratio was calculated. In parallel, neopterin concentrations were measured by ELISA. Tryptophan concentrations (median, interquartile range: 43.5, 31.2-56.3 microM) were lower in patients with gynecological cancer compared to healthy individuals of similar age (53.5, 47.0-64.2 microM; P<0.05). Kynurenine concentrations (median: 1.91 vs. 1.73 microM in controls) and kyn/trp (median: 41 vs. 35 micromol/mmol in controls) were slightly higher in patients, but not significantly different. Neopterin concentrations were significantly higher in patients (median: 10.8 vs. 7.0 nM in controls; P<0.05) and correlated with the kynurenine per tryptophan ratio (r(s)=0.555; P<0.02). In conclusion, tryptophan degradation is detectable in patients with gynecological cancer. The relationship between kyn/trp and neopterin concentrations indicates that cellular immune activation rather than tumor-mediated IDO-activity is responsible (228 words). |
| | |
Authors:
|
Katharina Schroecksnadel; Christiana Winkler; Lothar C Fuith; Dietmar Fuchs |
Related Documents
:
|
15025107 - Glucuronidation and susceptibility to chemical carcinogenesis. 6663147 - Clinicopathologic study of adenosquamous carcinoma of the uterine cervix. 14569767 - Relevance of cobb method in progressing sagittal plane spinal deformity. 3514427 - Immunohistochemical detection of laminin in 98 human breast carcinomas: a light and ele... 23447357 - Estrogen, progesterone, and her-2 receptor immunostaining in cytology: the effect of va... 22750847 - Ddx5 regulates dna replication and is required for cell proliferation in a subset of br... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-12-08 |
Journal Detail:
|
Title: Cancer letters Volume: 223 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2005 Jun |
Date Detail:
|
Created Date: 2005-05-17 Completed Date: 2005-07-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
|
Languages: eng Pagination: 323-9 Citation Subset: IM |
Affiliation:
|
Institute of Medical Chemistry and Biochemistry, Innsbruck Medical University, Fritz Pregl Strasse 3, A-6020 Innsbruck, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Age Factors Case-Control Studies Chromatography, High Pressure Liquid Female Genital Neoplasms, Female / immunology*, physiopathology* Humans Immunity, Cellular* Indoleamine-Pyrrole 2,3,-Dioxygenase Kynurenine / blood* Middle Aged Neopterin / blood Tryptophan / blood, metabolism* Tryptophan Oxygenase / pharmacology |
| Chemical | |
Reg. No./Substance:
|
343-65-7/Kynurenine; 670-65-5/Neopterin; 73-22-3/Tryptophan; EC 1.13.11.11/Tryptophan Oxygenase; EC 1.13.11.42/Indoleamine-Pyrrole 2,3,-Dioxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interactions between human colon carcinoma cells, fibroblasts and monocytic cells in coculture--regu...
Next Document: The inhibitor of apoptosis (IAP) survivin is expressed in human testicular germ cell tumors and norm...