Document Detail

Trypsin-like protease of mites: purification and characterization of trypsin-like protease from mite faecal extract Dermatophagoides farinae. Relationship between trypsin-like protease and Der f III.
MedLine Citation:
PMID:  10779309     Owner:  NLM     Status:  MEDLINE    
A serine protease from mite faecal extract, Dermatophagoides farinae, was purified using DEAE-Sephacel anion exchange chromatography and Superdex 75 pg gel chromatography. The molecular weight of this protease was 34 kD on SDS-PAGE under reducing conditions. The optimal pH and temperature of the protease were 8.0 and 47 degrees C, respectively. In addition, this protease cleaved arginyl or lysyl residue containing substrates selectively and was only inhibited by aprotinin, FUT-175, and soy bean trypsin inhibitor and not by chymostatin, E-64 and iodoacetic acid. These results show that our purified serine protease belongs to the trypsin-type. Purified trypsin-like protease was shown to be allergenic by enzyme-linked immunosorbent assay. Antigenicity of trypsin-like protease was completely different from those of Der f I and Der f II. Both, 20 N-terminal amino acid sequence and amino acid compositions of the purified protease were very similar to those of Der f III. Good similarities were found between trypsin-like protease and Der f III concerning physicochemical properties such as molecular weight on SDS-PAGE and ammonium sulphate solubility. Summarizing the above data, it can be concluded that a trypsin-like protease from mite faecal extract is actually the Der f III allergen and that it may be involved in the digestive process of the mite as it was found not in mite body but in mite faeces.
T Ando; R Homma; Y Ino; G Ito; A Miyahara; T Yanagihara; H Kimura; S Ikeda; H Yamakawa; M Iwaki
Related Documents :
2384149 - 3,4-dichloroisocoumarin, a serine protease inhibitor, inactivates glycogen phosphorylas...
5908969 - Inositol deficiency resulting in death: an explanation of its occurrence in neurospora ...
19090719 - Determination of three amino acids causing alteration of proteolytic activities of stap...
16955069 - Targeting proteases: successes, failures and future prospects.
20630859 - Isolation, cdna cloning, and structure-based functional characterization of oryctin, a ...
1782759 - Collagenolytic activity of crustacean midgut serine proteases: comparison with the bact...
8356929 - Proteases--structures, mechanism and inhibitors.
18437319 - Assays for beta-lactamase activity and inhibition.
9642219 - Regulation of the neutrophil respiratory burst oxidase. identification of an activation...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  23     ISSN:  0954-7894     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  1993 Sep 
Date Detail:
Created Date:  2000-06-22     Completed Date:  2000-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  777-84     Citation Subset:  IM    
Section of Allergology, Research Laboratories, Torii & Co. Ltd, Ichikawa, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Allergens / chemistry,  immunology,  isolation & purification*
Amino Acid Sequence
Amino Acids / analysis
Aprotinin / pharmacology
Chromatography, Gel
Chromatography, Ion Exchange
Digestive System / enzymology
Enzyme-Linked Immunosorbent Assay
Feces / enzymology
Guanidines / pharmacology
Hydrogen-Ion Concentration
Middle Aged
Mites / enzymology*
Molecular Sequence Data
Molecular Weight
Sequence Alignment
Sequence Homology, Amino Acid
Serine Endopeptidases / immunology,  isolation & purification*
Serine Proteinase Inhibitors / pharmacology
Substrate Specificity
Trypsin Inhibitors / pharmacology
Reg. No./Substance:
0/Allergens; 0/Amino Acids; 0/Guanidines; 0/Serine Proteinase Inhibitors; 0/Trypsin Inhibitors; 81525-10-2/nafamostat; 9087-70-1/Aprotinin; EC 3.4.21.-/Der f III allergen; EC 3.4.21.-/Serine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Formation of phosphatidylinositol-4-phosphate in human peripheral blood eosinophils.
Next Document:  Treatment of steroid-dependent asthma with recombinant interferon-gamma.