| Trypsin inhibitors from bottle gourd (Lagenaria leucantha Rusby var. Depressa Makino) seeds. Purification and amino acid sequences. | |
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MedLine Citation:
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PMID: 1562585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two almost identical trypsin isoinhibitors, LLDTI-I and LLDTI-II, from bottle gourd (Lagenaria leucantha Rusby var. Depressa Makino) seeds were purified by acetone precipitation, gel filtration and reversed phase chromatography. LLDTI-I and LLDTI-II consist of 30 and 29 amino acid residues, respectively, and have identical sequences, except that LLDTI-I has one additional pyroglutamic acid residue at N-terminus. Both proteins are strong inhibitors of bovine trypsin, with Ki values of 2.4.10(-10) M (LLDTI-I) and 9.6.10(-11) M (LLDTI-II). Amino acid sequences are as follows: [sequence: see text] |
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Authors:
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M Matsuo; N Hamato; R Takano; K Kamei-Hayashi; Y Yasuda-Kamatani; K Nomoto; S Hara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1120 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-05-20 Completed Date: 1992-05-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 187-92 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Molecular Sequence Data Peptide Mapping Plants / chemistry* Seeds Sequence Alignment Trypsin Inhibitors / chemistry, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Trypsin Inhibitors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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