Document Detail

Trypsin inhibitors from bottle gourd (Lagenaria leucantha Rusby var. Depressa Makino) seeds. Purification and amino acid sequences.
MedLine Citation:
PMID:  1562585     Owner:  NLM     Status:  MEDLINE    
Two almost identical trypsin isoinhibitors, LLDTI-I and LLDTI-II, from bottle gourd (Lagenaria leucantha Rusby var. Depressa Makino) seeds were purified by acetone precipitation, gel filtration and reversed phase chromatography. LLDTI-I and LLDTI-II consist of 30 and 29 amino acid residues, respectively, and have identical sequences, except that LLDTI-I has one additional pyroglutamic acid residue at N-terminus. Both proteins are strong inhibitors of bovine trypsin, with Ki values of 2.4.10(-10) M (LLDTI-I) and 9.6.10(-11) M (LLDTI-II). Amino acid sequences are as follows: [sequence: see text]
M Matsuo; N Hamato; R Takano; K Kamei-Hayashi; Y Yasuda-Kamatani; K Nomoto; S Hara
Related Documents :
10978735 - Purification and characterization of two hemolysins from stichodactyla helianthus.
3812085 - Mechanism of synthesis and localization of mitochondrial and cytosolic fumarases in rat...
2065665 - Xubf and rib 1 are both required for formation of a stable polymerase i promoter comple...
8881285 - Spermatogenesis of the lizard lacerta vivipara: histological studies and amino acid seq...
6509925 - A comparative study of the size-heterogeneous high mannose oligosaccharides of some ins...
8281325 - Induction of fictive locomotion by sulphur-containing amino acids in an in vitro newbor...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1120     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-05-20     Completed Date:  1992-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  187-92     Citation Subset:  IM    
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Molecular Sequence Data
Peptide Mapping
Plants / chemistry*
Sequence Alignment
Trypsin Inhibitors / chemistry,  isolation & purification*
Reg. No./Substance:
0/Trypsin Inhibitors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Microsomal retinal synthesis: retinol vs. holo-CRBP as substrate and evaluation of NADP, NAD and NAD...
Next Document:  Biophysical characterization of alpha-crystallin aggregates: validation of the micelle hypothesis.