Document Detail


Trypsin from the viscera of Bogue (Boops boops): isolation and characterisation.
MedLine Citation:
PMID:  19898990     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Trypsin from the viscera of Bogue (Boops boops) was purified to homogeneity by precipitation with ammonium sulphate, Sephadex G-100 gel filtration and Mono Q-Sepharose anion exchange chromatography, with an 8.5-fold increase in specific activity and 36% recovery. The molecular weight of the purified enzyme was estimated to be 23 kDa by SDS-PAGE and size exclusion chromatography. The purified trypsin appeared as a single band on native-PAGE and zymography staining. The purified enzyme showed esterase-specific activity on N-α-benzoyl-L-arginine ethyl ester (BAEE) and amidase activity on N-α-benzoyl-DL-arginine-p-nitroanilide (BAPNA). The optimum pH and temperature for the enzyme activity, after 10 min incubation, were pH 9.0 and 55°C, respectively, using BAPNA as a substrate. The trypsin kinetic constants Km and kcat on BAPNA were 0.13 mM and 1.56 s(-1), respectively, while the catalytic efficiency kcat/Km was 12 s(-1) mM(-1). Biochemical characterisation of B. boops trypsin showed that this enzyme can be used as a possible biotechnological tool in the fish processing and food industries.
Authors:
Ahmed Barkia; Ali Bougatef; Rim Nasri; Emna Fetoui; Rafik Balti; Moncef Nasri
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-08
Journal Detail:
Title:  Fish physiology and biochemistry     Volume:  36     ISSN:  1573-5168     ISO Abbreviation:  Fish Physiol. Biochem.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955049     Medline TA:  Fish Physiol Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  893-902     Citation Subset:  IM    
Affiliation:
Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038, Sfax, Tunisia.
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