Document Detail

Trypsin and aminopeptidase gene expression is affected by age and food composition in Anopheles gambiae.
MedLine Citation:
PMID:  8995788     Owner:  NLM     Status:  MEDLINE    
The effects of age and food composition on the expression of trypsin and aminopeptidase genes in the Anopheles gambiae gut were investigated. No trypsin mRNA was detected in the gut of newly eclosed females, but this mRNA accumulated to relatively high levels within the first day of life. In contrast, low, but significant trypsin enzyme activity was observed in newly eclosed females. Subcellular fractionation experiments suggested that abdominal distention induces the secretion of the enzyme into the lumen. Blood, but not a protein-free meal, induced the accumulation of new trypsin mRNA and enzyme. Unlike trypsin, substantial aminopeptidase activity was detected in newly eclosed and in older, sugar fed mosquitoes. Moreover, the subcellular localization of aminopeptidase did not change appreciably with food ingestion, and the early increase of enzyme activity was independent of food composition.
F J Lemos; A J Cornel; M Jacobs-Lorena
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Insect biochemistry and molecular biology     Volume:  26     ISSN:  0965-1748     ISO Abbreviation:  Insect Biochem. Mol. Biol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1997-02-06     Completed Date:  1997-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207282     Medline TA:  Insect Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  651-8     Citation Subset:  IM    
Universidade Estadual do Norte Fluminense, Centro de Biochiencias e Biotecnologia, R.J., Brazil.
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MeSH Terms
Age Factors
Aminopeptidases / genetics,  metabolism*
Animal Feed
Anopheles / enzymology*,  genetics
Chromosome Mapping
DNA, Complementary
Gene Expression
RNA, Messenger
Time Factors
Trypsin / genetics,  metabolism*
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger; EC 3.4.11.-/Aminopeptidases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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