| Trypanosoma cruzi: growth requirements at different temperature in fetal bovine serum or peptide supplemented media. | |
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MedLine Citation:
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PMID: 3301389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An enriched synthetic medium with low molecular weight peptides allows Trypanosoma cruzi epimastigotes to grow at 26-37 C. Using this medium, the growth requirements of T. cruzi were compared at different temperatures. When supplemented with fetal bovine serum or serum peptides, nine amino acids were absolutely required from the first passage, while additional amino acids and amino acid precursors were needed to support growth during a second passage. Five amino acids (beta-alanine, glutamine, cysteine, ornithine, and threonine) were also required absolutely at temperatures ranging between 30 and 37 C. Nine vitamins were needed at all temperatures, while ascorbic acid and ergocalciferol were not necessary at any temperature. The remaining amino acids and vitamins showed a variable role as growth factors depending on the temperature increase. In peptide supplemented media, requirements for amino acids and their precursors, as well as vitamins and nucleotides, increased markedly when compared with the protein supplemented medium. A peptide composed of one glutamic acid, two alanines, and one lysine can substitute for serum for trypanosomal growth at all temperatures. Several minimum media have been prepared in which epimastigote forms of T. cruzi can grow at 26-37 C for more than 10 passages. |
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Authors:
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J A O'Daly; M B Rodríguez; G Garlin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental parasitology Volume: 64 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1987 Aug |
Date Detail:
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Created Date: 1987-09-14 Completed Date: 1987-09-14 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 78-87 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology Culture Media Fetal Blood / physiology Hydrogen-Ion Concentration Peptides / pharmacology* Temperature Trypanosoma cruzi / growth & development* Vitamins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 0/Peptides; 0/Vitamins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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