| Trypanosoma cruzi detection in blood by xenodiagnosis and polymerase chain reaction in the wild rodent Octodon degus. | |
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MedLine Citation:
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PMID: 17297043 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We detected Trypanosoma cruzi in blood samples of the wild rodent Octodon degus by xenodiagnosis and a polymerase chain reaction (PCR) using the domestic and wild vectors of Chagas disease, Triatoma infestans and Mepraia spinolai, respectively. We captured 35 rodents and extracted DNA from blood samples and intestinal contents of vectors fed on O. degus. Our results indicate that the percentage of rodents naturally infected with T. cruzi depends on the biologic sample used for PCR and on the vector species for xenodiagnosis. The PCR with blood samples did not detect T. cruzi DNA, but the PCR with intestinal contents showed that both vectors were positive for T. cruzi. The PCR performed with M. spinolai intestinal contents detected four times more T. cruzi-positive O. degus than the PCR with Triatoma infestans intestinal contents (22.9% and 5.7%, respectively). We report the improvement of T. cruzi detection in sylvatic animals by a combination of PCR and xenodiagnosis using sylvatic vectors, especially in disease-endemic areas with low parasitemias in mammals. |
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Authors:
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Ricardo Campos; Carezza Botto-Mahan; Sylvia Ortiz; Mariana Acuña; Pedro E Cattan; Aldo Solari |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of tropical medicine and hygiene Volume: 76 ISSN: 0002-9637 ISO Abbreviation: Am. J. Trop. Med. Hyg. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-02-13 Completed Date: 2007-04-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370507 Medline TA: Am J Trop Med Hyg Country: United States |
Other Details:
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Languages: eng Pagination: 324-6 Citation Subset: AIM; IM |
Affiliation:
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Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chagas Disease / blood, epidemiology, parasitology, veterinary* Chile / epidemiology DNA, Protozoan / chemistry, genetics Feces / parasitology Octodon / blood, parasitology* Polymerase Chain Reaction / veterinary Rodent Diseases / blood, epidemiology, parasitology* Seroepidemiologic Studies Triatoma / parasitology* Trypanosoma cruzi / isolation & purification* Xenodiagnosis / veterinary |
| Chemical | |
Reg. No./Substance:
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0/DNA, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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