| Trypanosoma congolense: B-lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle. | |
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MedLine Citation:
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PMID: 8654538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trypanosomiasis is a serious constraint to livestock production in sub-Saharan Africa. Some breeds of cattle are genetically more resistant to the pathogenic effects of trypanosome infection. We measured B-cell activation and the quantity and isotype of antibody produced at the cellular level in six trypanotolerant N'Dama and five trypanosusceptible Boran cattle. The frequencies of spleen cells secreting total and parasite-specific IgM and IgG were measured prior to and 16, 28, and 35 days after a primary challenge with Trypanosoma congolense. Boran cattle had higher frequencies of splenic cells secreting IgM specific for trypanosome-derived variable surface glycoprotein (VSG), cysteine protease (congopain, CP), and heat shock protein (hsp 70/BiP) and the nonparasite antigen, ovalbumin, than did N'Dama cattle. In contrast, the number of VSG-specific IgG-secreting cells was significantly greater in N'Dama than in Boran cattle. During infection, low titers of anti-VSG IgM were detected transiently in the serum of all animals. However, N'Dama had significantly more VSG-specific IgG in blood than Boran during infection. The peripheral blood mononuclear cell population of N'Dama cattle contained a higher percentage of surface IgM-positive B-cells prior to and throughout infection than were found in the blood of Boran. In addition, during infection N'Dama cattle had more circulating lymphocytes that could be activated in vitro to undergo differentiation into IgM- and IgG-secreting cells. These findings demonstrate differences in the frequency of trypanosome-specific antibody-secreting cells in the spleen and in the activation state of B-cells in the blood between N'Dama and Boran cattle during a primary infection with T. congolense. |
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Authors:
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K A Taylor; V Lutje; D Kennedy; E Authié; A Boulangé; L Logan-Henfrey; B Gichuki; G Gettinby |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental parasitology Volume: 83 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-08-01 Completed Date: 1996-08-01 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 106-16 Citation Subset: IM |
Affiliation:
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International Livestock Research Institute, Nairobi, Kenya. k.taylor@cgnet.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Anemia / immunology, veterinary Animals Antibody-Producing Cells / immunology B-Lymphocytes / immunology* Cattle Cysteine Endopeptidases / immunology Disease Susceptibility Enzyme-Linked Immunosorbent Assay Flow Cytometry Hematocrit / veterinary Immunity, Innate Immunoglobulin G / biosynthesis, blood Immunoglobulin M / biosynthesis, blood Lymphocyte Activation Parasitemia / immunology, veterinary Species Specificity Spleen / cytology, immunology Trypanosoma congolense / immunology* Trypanosomiasis, African / immunology, veterinary Trypanosomiasis, Bovine / immunology* Variant Surface Glycoproteins, Trypanosoma / immunology |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; 0/Immunoglobulin M; 0/Variant Surface Glycoproteins, Trypanosoma; EC 3.4.22.-/Cysteine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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