Document Detail


Trypanosoma brucei FKBP12 differentially controls motility and cytokinesis in procyclic and bloodstream forms.
MedLine Citation:
PMID:  23104568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
FKBP12 proteins are able to inhibit TOR kinases or calcineurin phosphatases upon binding of rapamycin or FK506 drugs, respectively. The Trypanosoma brucei FKBP12 homologue (TbFKBP12) was found to be a cytoskeleton-associated protein with specific localization in the flagellar pocket area of the bloodstream form. In the insect procyclic form, RNA interference-mediated knockdown of TbFKBP12 affected motility. In bloodstream cells, depletion of TbFKBP12 affected cytokinesis and cytoskeleton architecture. These last effects were associated with the presence of internal translucent cavities limited by an inside-out configuration of the normal cell surface, with a luminal variant surface glycoprotein coat lined up by microtubules. These cavities, which recreated the streamlined shape of the normal trypanosome cytoskeleton, might represent unsuccessful attempts for cell abscission. We propose that TbFKBP12 differentially affects stage-specific processes through association with the cytoskeleton.
Authors:
Anaïs Brasseur; Brice Rotureau; Marjorie Vermeersch; Thierry Blisnick; Didier Salmon; Philippe Bastin; Etienne Pays; Luc Vanhamme; David Pérez-Morga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-26
Journal Detail:
Title:  Eukaryotic cell     Volume:  12     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-30     Completed Date:  2013-07-16     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  168-81     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Parasitology, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Cell Movement
Cytokinesis*
DNA, Kinetoplast / metabolism
DNA, Protozoan / metabolism
Flagella / metabolism,  ultrastructure
Gene Knockdown Techniques
Microtubules / metabolism
Protein Transport
Protozoan Proteins / genetics,  physiology*
RNA, Small Interfering / genetics
Tacrolimus Binding Protein 1A / genetics,  metabolism*
Trypanosoma brucei brucei / enzymology*,  growth & development,  ultrastructure
Chemical
Reg. No./Substance:
0/DNA, Kinetoplast; 0/DNA, Protozoan; 0/Protozoan Proteins; 0/RNA, Small Interfering; EC 5.2.1.-/Tacrolimus Binding Protein 1A
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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