Document Detail

Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells.
MedLine Citation:
PMID:  16289356     Owner:  NLM     Status:  MEDLINE    
Homologous recombination acts in the repair of cellular DNA damage and can generate genetic variation. Some of this variation provides a discrete purpose in the cell, although it can also be genome-wide and contribute to longer-term natural selection. In Trypanosoma brucei, a eukaryotic parasite responsible for sleeping sickness disease in sub-Saharan Africa, homologous recombination acts to catalyse antigenic variation, an immune evasion strategy involving switches in variant surface glycoprotein. In addition, T. brucei can undergo genetic exchange by homologous recombination in the tsetse vector, and some evidence suggests that this occurs by meiosis. Here, we show that T. brucei, Trypanosoma cruzi and Leishmania major each contain a single copy gene whose product is highly related to the eukaryotic meiosis-specific protein Dmc1, which is structurally and functionally related to Rad51. We show that T. brucei DMC1 is transcribed in the bloodstream stage of the parasite, where the gene can be mutated by reverse genetic disruption. DMC1 mutation does not, however, result in detectable alterations in DNA repair, recombination or antigenic variation efficiency in this life cycle stage.
Chris Proudfoot; Richard McCulloch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-26
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  145     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-03-23     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  245-53     Citation Subset:  IM    
The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK.
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MeSH Terms
Amino Acid Sequence
Antigenic Variation*
DNA Repair*
Gene Expression
Leishmania major / genetics
Molecular Sequence Data
Mutagenesis, Insertional
Protozoan Proteins / genetics,  physiology*
Rad51 Recombinase / genetics
Recombination, Genetic*
Sequence Homology, Amino Acid
Trypanosoma brucei brucei / genetics,  immunology,  physiology*
Trypanosoma cruzi / genetics
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Protozoan Proteins; EC 2.7.7.-/Rad51 Recombinase

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