| Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells. | |
| | |
MedLine Citation:
|
PMID: 16289356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Homologous recombination acts in the repair of cellular DNA damage and can generate genetic variation. Some of this variation provides a discrete purpose in the cell, although it can also be genome-wide and contribute to longer-term natural selection. In Trypanosoma brucei, a eukaryotic parasite responsible for sleeping sickness disease in sub-Saharan Africa, homologous recombination acts to catalyse antigenic variation, an immune evasion strategy involving switches in variant surface glycoprotein. In addition, T. brucei can undergo genetic exchange by homologous recombination in the tsetse vector, and some evidence suggests that this occurs by meiosis. Here, we show that T. brucei, Trypanosoma cruzi and Leishmania major each contain a single copy gene whose product is highly related to the eukaryotic meiosis-specific protein Dmc1, which is structurally and functionally related to Rad51. We show that T. brucei DMC1 is transcribed in the bloodstream stage of the parasite, where the gene can be mutated by reverse genetic disruption. DMC1 mutation does not, however, result in detectable alterations in DNA repair, recombination or antigenic variation efficiency in this life cycle stage. |
| | |
Authors:
|
Chris Proudfoot; Richard McCulloch |
Related Documents
:
|
8546446 - Development of recombinant baculoviruses for insect control. 9140916 - Molecular population genetic analysis of a streptococcus pyogenes bacteriophage-encoded... 7883086 - Genetic stability of protein expression systems in yeast. 1894606 - Carboxyl terminus region modulates catalytic activity of recombinant maize aldolase. 11402146 - Missense and splice site mutations in tau associated with ftdp-17: multiple pathogenic ... 8980256 - Genetic analysis of beta-thalassemia intermedia in israel: diversity of mechanisms and ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-26 |
Journal Detail:
|
Title: Molecular and biochemical parasitology Volume: 145 ISSN: 0166-6851 ISO Abbreviation: Mol. Biochem. Parasitol. Publication Date: 2006 Feb |
Date Detail:
|
Created Date: 2006-01-17 Completed Date: 2006-03-23 Revised Date: 2007-08-13 |
Medline Journal Info:
|
Nlm Unique ID: 8006324 Medline TA: Mol Biochem Parasitol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 245-53 Citation Subset: IM |
Affiliation:
|
The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Antigenic Variation* DNA Repair* Gene Expression Leishmania major / genetics Molecular Sequence Data Mutagenesis, Insertional Mutation Protozoan Proteins / genetics, physiology* Rad51 Recombinase / genetics Recombination, Genetic* Sequence Homology, Amino Acid Trypanosoma brucei brucei / genetics, immunology, physiology* Trypanosoma cruzi / genetics |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Protozoan Proteins; EC 2.7.7.-/Rad51 Recombinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Raised circulating corticosterone inhibits neuronal differentiation of progenitor cells in the adult...
Next Document: Upregulation of expression of the reticulocyte homology gene 4 in the Plasmodium falciparum clone Dd...