Document Detail


Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus.
MedLine Citation:
PMID:  21909398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. Here, we show that the pore-forming toxin HlyII of Bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. These findings suggest that the dye enters viable cells through the pores. To our knowledge, this is the first demonstration that trypan blue may enter viable cells. Consequently, the use of trypan blue staining as a marker of vital status should be interpreted with caution. The blue coloration does not necessarily indicate cell lysis, but may rather indicate pore formation in the cell membranes and more generally increased membrane permeability.
Authors:
Seav-Ly Tran; Andrea Puhar; Maud Ngo-Camus; Nalini Ramarao
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Publication Detail:
Type:  Journal Article     Date:  2011-09-06
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-12     Completed Date:  2012-01-05     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e22876     Citation Subset:  IM    
Affiliation:
INRA, Unité MICALIS, UMR 1319, Guyancourt, France.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / biosynthesis
Animals
Bacillus cereus / metabolism*
Bacterial Proteins / pharmacology*
Cell Membrane Permeability / drug effects
Cell Survival / drug effects
Coloring Agents / metabolism*
Hemolysin Proteins / pharmacology*
Macrophages / cytology,  drug effects,  metabolism*
Mice
Trypan Blue / metabolism*
Chemical
Reg. No./Substance:
0/ApxII toxin, bacteria; 0/Bacterial Proteins; 0/Coloring Agents; 0/Hemolysin Proteins; 56-65-5/Adenosine Triphosphate; 72-57-1/Trypan Blue
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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