Document Detail

Truncated hemoglobins in Frankia CcI3: effects of nitrogen source, oxygen concentration, and nitric oxide.
MedLine Citation:
PMID:  19767859     Owner:  NLM     Status:  MEDLINE    
Frankia strain CcI3 produces 2 truncated hemoglobins, HbN and HbO. Using ion-exchange chromatography, we characterized the expression of the relative amounts of HbN and HbO in -N (nitrogen-fixing) cultures and +N (nitrogen-supplemented) cultures. The -N cultures maintained an approximately constant ratio of HbO to HbN throughout the life of the culture, with HbO constituting 80%-85% of the total hemoglobin produced. In contrast, in +N cultures, HbN was observed to increase over time and HbO decreased. Total hemoglobin as a fraction of total protein was approximately constant throughout the growth phase in -N cultures, while it decreased somewhat in +N cultures. Subjecting -N cultures to a NO generator resulted in increased production of HbN, relative to the controls. Nitrite accumulated in +N cultures, but not in -N cultures. This suggests that the greater amount of HbN in +N cultures might be due to NO produced by the reduction of nitrite. The effects of O2 concentration were determined in +N cultures. Cultures grown in 1% O2 produced about 4 times more HbO than cultures grown in 20% O2. Overall, these results provide evidence for a role of HbN in NO oxidation and for a role of HbO in adaptation to low oxygen concentrations.
Vanessa Coats; Christa R Schwintzer; John D Tjepkema
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  55     ISSN:  1480-3275     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-09-21     Completed Date:  2009-11-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  867-73     Citation Subset:  IM    
School of Biology and Ecology, University of Maine, 5722 Deering Hall, Orono, ME 04469-5722, USA.
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MeSH Terms
Bacterial Proteins / chemistry,  genetics,  metabolism*
Frankia / chemistry,  genetics,  metabolism*
Gene Expression Regulation, Bacterial
Molecular Weight
Nitric Oxide / metabolism*
Nitrites / metabolism
Nitrogen / metabolism*
Oxygen / metabolism*
Truncated Hemoglobins / chemistry,  genetics,  metabolism*
Reg. No./Substance:
0/Bacterial Proteins; 0/Nitrites; 0/Truncated Hemoglobins; 10102-43-9/Nitric Oxide; 7727-37-9/Nitrogen; 7782-44-7/Oxygen

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