Document Detail

Truncated forms of the human prion protein in normal brain and in prion diseases.
MedLine Citation:
PMID:  7642585     Owner:  NLM     Status:  MEDLINE    
The cellular form of the prion protein (PrPc) is a glycoprotein anchored to the cell membrane by a glycosylphosphatidylinositol moiety. An aberrant form of PrPc that is partially resistant to proteases, PrPres, is a hallmark of prion diseases, which in humans include Cruetzfeldt-Jakob disease (CJD), Gerstmann-Sträussler-Scheinker syndrome, and fatal familial insomnia. We have characterized the major forms of PrP in normal and pathological human brains. A COOH-terminal fragment of PrPc, designated C1, is abundant in normal and CJD brains as well as in human neuroblastoma cells. Sequence analysis revealed that C1 contains alternative NH2 termini starting at His-111 or Met-112. Like PrPc, C1 is glycosylated, anchored to the cell membrane, and is heat-stable. Consistent with the lack of the NH2-terminal region of PrPc, C1 is more acidic than PrPc and does not bind heparin. An additional fragment longer than C1, designated C2, is present in substantial amounts in CJD brains. Like PrPres, C2 is resistant to proteases and is detergent-insoluble. Our data indicate that C1 is a major product of normal PrPc metabolism, generated by a cleavage that disrupts the neurotoxic and amyloidogenic region of PrP comprising residues 106-126. This region remains intact in C2, suggesting a role for C2 in prion diseases.
S G Chen; D B Teplow; P Parchi; J K Teller; P Gambetti; L Autilio-Gambetti
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  270     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-18     Completed Date:  1995-09-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  19173-80     Citation Subset:  IM    
Division of Neuropathology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
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MeSH Terms
Aged, 80 and over
Amino Acid Sequence
Brain Chemistry*
Middle Aged
Molecular Sequence Data
Neuroblastoma / chemistry
Peptide Fragments / analysis
Prion Diseases / metabolism*
Prions / analysis*,  chemistry
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Peptide Fragments; 0/Prions

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