| Truncated cystatin C in cerebrospiral fluid: Technical [corrected] artefact or biological process? | |
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MedLine Citation:
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PMID: 16021609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cystatin C, a low molecular weight cysteine proteinase inhibitor present in human body fluids at physiological concentrations, is more expressed in cerebrospinal fluid (CSF) than in plasma. Mass spectrometric characterization showed that after 3 months of storage of human CSF at -20 degrees C, cystatin C was cleaved in the peptide bond between R8 and L9 and lost its eight N-termini amino acids, whereas this cleavage did not occur when stored at -80 degrees C. This truncation occurred in all CSF samples studied irrespective of the underlying neurological status, indicating a storage-related artefact rather than a physiological or pathological processing of the protein. These results stress the importance of optimal preanalytical storage conditions of any sample prior to proteomics studies. |
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Authors:
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Odile Carrette; Pierre R Burkhard; Severine Hughes; Denis F Hochstrasser; Jean-Charles Sanchez |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proteomics Volume: 5 ISSN: 1615-9853 ISO Abbreviation: Proteomics Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-10 Completed Date: 2005-10-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 3060-5 Citation Subset: IM |
Affiliation:
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Biomedical Proteomics Research Group, Geneva University Hospital and Medical School, 1 rue Michel Servet, CH-1211 Geneva 4, Switzerland. Odile.Carrette@medecine.unige.ch |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Artifacts Cystatin C Cystatins / cerebrospinal fluid*, chemistry* Cysteine Proteinase Inhibitors / chemistry Electrophoresis, Polyacrylamide Gel Humans Mass Spectrometry / methods Protein Array Analysis Protein Structure, Tertiary Proteins / chemistry Proteomics / methods Specimen Handling Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/CST3 protein, human; 0/Cystatin C; 0/Cystatins; 0/Cysteine Proteinase Inhibitors; 0/Proteins |
| Comments/Corrections | |
Erratum In:
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Proteomics. 2005 Sep;5(14):3822 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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