| Truncated c-Myb expression in the human leukemia cell line TK-6. | |
MedLine Citation:
|
PMID: 9737692 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The c-MYB proto-oncogene encodes a transcription factor which plays an important role in hematopoiesis. We detected truncated c-MYB mRNA (2.0 kb) and c-Myb protein (55 kDa) in the TK-6 cell line, which was established from a patient with chronic myelogenous leukemia in T cell blast crisis. Mutated c-MYB cDNA clone (WTK-1) was isolated from a TK-6 cell cDNA library and sequenced in its entirety. Compared with the wild-type human c-MYB sequence, the WTK-1 sequence diverged at the 3' ends of exons 9. A termination codon was present as the second codon downstream from the point of divergence and was followed by a previously unknown rearranged sequence. The conceptual protein encoded by WTK-1 (Myb(TK-6)) comprises 402 amino acids and lacks the negative regulatory domain of the normal c-Myb, reminiscent of the activated form of Myb protein. Luciferase reporter assay in NIH3T3 cells showed that the expression vector encoding Myb(TK-6) stimulated Myb-regulated mim-1 promoter more effectively than that encoding wild-type human c-Myb, suggesting that Myb(TK-6) is functional as a transcription factor, and thus as a potential transforming protein. Southern blot and mutant allele-specific polymerase chain reaction analyses showed that the same rearrangement of the c-MYB gene in TK-6 was present in late, but not in early, specimens obtained from the patient, indicating that this mutation had been acquired during disease progression. |
Authors:
|
A Tomita; T Watanabe; H Kosugi; H Ohashi; T Uchida; T Kinoshita; S Mizutani; T Hotta; T Murate; M Seto; H Saito |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Leukemia Volume: 12 ISSN: 0887-6924 ISO Abbreviation: Leukemia Publication Date: 1998 Sep |
Date Detail:
|
Created Date: 1998-09-24 Completed Date: 1998-09-24 Revised Date: 2013-03-04 |
Medline Journal Info:
|
Nlm Unique ID: 8704895 Medline TA: Leukemia Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1422-9 Citation Subset: IM |
Affiliation:
|
First Department of Internal Medicine, Nagoya University School of Medicine, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Base Sequence Blast Crisis / metabolism Chromosomes, Human, Pair 6 / genetics* DNA, Complementary / genetics DNA, Neoplasm / genetics HL-60 Cells HeLa Cells Humans Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism* Male Molecular Sequence Data Proto-Oncogene Proteins / genetics*, metabolism Proto-Oncogene Proteins c-myb Sequence Analysis, DNA Trans-Activators / genetics*, metabolism Transcriptional Activation Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Complementary; 0/DNA, Neoplasm; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/Trans-Activators |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Different patterns of homozygous p16INK4A and p15INK4B deletions in childhood acute lymphoblastic le...
Next Document: Models of lineage switching in hematopoietic development: a new myeloid-committed eosinophil cell li...