Document Detail


Trp1, a candidate protein for the store-operated Ca(2+) influx mechanism in salivary gland cells.
MedLine Citation:
PMID:  10652333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The trp gene family has been proposed to encode the store-operated Ca(2+) influx (SOC) channel(s). This study examines the role of Trp1 in the SOC mechanism of salivary gland cells. htrp1, htrp3, and Trp1 were detected in the human submandibular gland cell line (HSG). HSG cells stably transfected with htrp1alpha cDNA displayed (i) a higher level of Trp1, (ii) a 3-5-fold increase in SOC (thapsigargin-stimulated Ca(2+) influx), determined by [Ca(2+)](i) and Ca(2+)-activated K(+) channel current measurements, and (iii) similar basal Ca(2+) permeability, and inhibition of SOC by Gd(3+) but not by Zn(2+), as compared with control cells. Importantly, (i) transfection of HSG cells with antisense trp1alpha cDNA decreased endogenous Trp1 level and significantly attenuated SOC, and (ii) transfection of HSG cells with htrp3 cDNA did not increase SOC. These data demonstrate an association between Trp1 and SOC and strongly suggest that Trp1 is involved in this mechanism in HSG cells. Consistent with this suggestion, Trp1 was detected in the plasma membrane region, the proposed site of SOC, of acinar and ductal cells in intact rat submandibular glands. Based on these aggregate data, we propose Trp1 as a candidate protein for the SOC mechanism in salivary gland cells.
Authors:
X Liu; W Wang; B B Singh; T Lockwich; J Jadlowiec; B O'Connell; R Wellner; M X Zhu; I S Ambudkar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  275     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-02     Completed Date:  2000-03-02     Revised Date:  2012-02-07    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3403-11     Citation Subset:  IM    
Affiliation:
Secretory Physiology Section, Gene Therapy and Therapeutics Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Calcium Channels / genetics,  metabolism*
Cells, Cultured
Humans
Ion Transport
Oligonucleotides, Antisense
Rats
Salivary Glands / metabolism*
TRPC Cation Channels
Transfection
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/Oligonucleotides, Antisense; 0/TRPC Cation Channels; 0/transient receptor potential cation channel, subfamily C, member 1; 7440-70-2/Calcium
Comments/Corrections
Erratum In:
J Biol Chem 2000 Mar 31;275(13):9890-1

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